{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T11:15:38Z","timestamp":1780398938854,"version":"3.54.1"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,3,30]],"date-time":"2021-03-30T00:00:00Z","timestamp":1617062400000},"content-version":"vor","delay-in-days":88,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002799","name":"Capital Medical University","doi-asserted-by":"publisher","award":["FCYY201720"],"award-info":[{"award-number":["FCYY201720"]}],"id":[{"id":"10.13039\/501100002799","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002799","name":"Capital Medical University","doi-asserted-by":"publisher","award":["PYZ2018010"],"award-info":[{"award-number":["PYZ2018010"]}],"id":[{"id":"10.13039\/501100002799","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. To explore the function of miR\u2010543 in endometrial cells and the possible mechanism of regulating the occurrence and development of intrauterine adhesion. <jats:italic>Method<\/jats:italic>. Endometrial epithelial cells and endometrial adenocarcinoma cells were transfected with miR\u2010543 mimics and miR\u2010543 inhibitor as the experimental group and were tested with the control group, using the CCK\u20108 method, scratch test, and Transwell assay, and flow cytometry was used to detect the proliferation, migration, invasion, and apoptosis of cells. RT\u2010qPCR and Western blot were used to detect the expression of corresponding mRNA and protein. <jats:italic>Results<\/jats:italic>. After the overexpression of miR\u2010543, endometrial epithelial cells and endometrial adenocarcinoma cells have reduced migratory, proliferative, and invasive capabilities, while the apoptosis rate has increased significantly. The mRNA expression of <jats:italic>CDH2<\/jats:italic>, <jats:italic>COL16A1<\/jats:italic>, <jats:italic>vimentin<\/jats:italic>, <jats:italic>\u03b1-SMA<\/jats:italic> and <jats:italic>fibronectin<\/jats:italic> decreased, and the protein expression of CDH2, vimentin, and <jats:italic>\u03b1<\/jats:italic>\u2010SMA also decreased, while the mRNA and protein expression of CDH1 increased. The result after interfering with miR\u2010543 is opposite, and luciferase reporter gene confirms that <jats:italic>CDH2<\/jats:italic> is the target gene of miR\u2010543. <jats:italic>Conclusion<\/jats:italic>. During the formation of intrauterine adhesions, the expression of <jats:italic>CDH2<\/jats:italic>, <jats:italic>COL16A1<\/jats:italic>, <jats:italic>vimentin<\/jats:italic>, and <jats:italic>\u03b1-SMA<\/jats:italic> may be inhibited by the high expression of miR\u2010543, which may affect the degree of fibrosis and collagen content in the intrauterine adhesions, thereby inhibiting the occurrence and development of intrauterine adhesions.<\/jats:p>","DOI":"10.1155\/2021\/5559102","type":"journal-article","created":{"date-parts":[[2021,3,30]],"date-time":"2021-03-30T23:20:10Z","timestamp":1617146410000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["miR\u2010543 Inhibits the Occurrence and Development of Intrauterine Adhesion by Inhibiting the Proliferation, Migration, and Invasion of Endometrial Cells"],"prefix":"10.1155","volume":"2021","author":[{"given":"Xin","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9428-7561","authenticated-orcid":false,"given":"Hua","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,30]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/589296"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmig.2017.09.006"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmt045"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-3804(05)60129-6"},{"key":"e_1_2_9_5_2","first-page":"529","article-title":"Expression of miR-195 in intrauterine adhesions and its relationship with TGF-\u03b21\/Smads,FGF2\/FHFR1\/ERK pathways","volume":"2020","author":"LG W. 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