{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:40:57Z","timestamp":1747190457669,"version":"3.40.5"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,5,22]],"date-time":"2021-05-22T00:00:00Z","timestamp":1621641600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Minhang District Central Hospital of Shanghai"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Computational and Mathematical Methods in Medicine"],"published-print":{"date-parts":[[2021,5,22]]},"abstract":"<jats:p>Aim. To research the molecular mechanism of ghrelin in apoptosis, migratory, and invasion of gastric cancer (GC) cells. Methods. After GC AGS cells were handled with ghrelin (10\u20138\u2009M), cyclooxygenase-2 inhibitor NS398 (100\u2009\u03bcM), and Akt inhibitor perifosine (10uM), the rates of apoptosis were detected by TUNEL assay and flow cytometry assay. We assessed the expressions of PI3K, p-Akt, and COX-2 proteins by making use of Western blot analysis. The cell migratory and invasion were detected by using wound-healing and transwell analysis. Results. The migratory and invasion were increased in ghrelin-treated cells, while the rates of apoptosis were decreased. GC AGS cells treated with ghrelin showed an increase in protein expression of p-Akt, PI3K, and COX-2. After cells were treated with Akt inhibitor perifosine, the protein expression of p-Akt, PI3K, and COX-2 and the cell migratory, invasion, and apoptosis were partly recovered. After cells were treated with cyclooxygenase-2 inhibitor NS398, the protein expression of COX-2 and the cell migratory and invasion were decreased, while the rates of apoptosis were increased. Conclusion. Ghrelin regulates cell migration, invasion, and apoptosis in GC cells through targeting PI3K\/Akt\/COX-2. Ghrelin increases the expression of COX-2 in GC cells by targeting PI3K\/Akt. Ghrelin is suggested to be one of the molecular targets in GC.<\/jats:p>","DOI":"10.1155\/2021\/5576808","type":"journal-article","created":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T20:20:08Z","timestamp":1621887608000},"page":"1-9","source":"Crossref","is-referenced-by-count":3,"title":["Ghrelin Regulates Cyclooxygenase-2 Expression and Promotes Gastric Cancer Cell Progression"],"prefix":"10.1155","volume":"2021","author":[{"given":"Huanqing","family":"Li","sequence":"first","affiliation":[{"name":"Endoscopy Center, Minhang District Central Hospital of Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5419-0899","authenticated-orcid":true,"given":"Xiaohong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Endoscopy Center, Minhang District Central Hospital of Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5309-8975","authenticated-orcid":true,"given":"Li","family":"Feng","sequence":"additional","affiliation":[{"name":"Endoscopy Center, Minhang District Central Hospital of Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21442"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i38.13804"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-405X.2006.00424.x"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0463.2003.1111001.x"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.15.4907"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.15.2731"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1038\/45230"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.040600"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3568"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-8-170"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-1596"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1002\/path.4552"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.09.043"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.2147\/dddt.s89096"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-002-1_6"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.3109\/07388551.2015.1128876"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-406-9:183"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1111\/jgh.15037"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1159\/000321703"},{"issue":"10","key":"20","first-page":"e499","article-title":"Cancer treatment. 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