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Although tyrosine kinase inhibitors (TKIs) have restored and maintained the quality of life of patients with CML, an important minority of patients become resistant to first\u2010and\u2010second\u2010generation TKIs and require an alternative treatment. The K562 cell (Ph+, p53\u2010\/\u2010) line was treated with Vit E TPGS 1000 (20\u201380\u2009<jats:italic>\u03bc<\/jats:italic>M) only or with other products of interest (e.g., antioxidant N\u2010acetylcysteine (NAC), specific JNK and caspase\u20103 inhibitor SP600125, and NSCSI, respectively) for 24\u2009h at 37\u00b0C. Cells were analyzed by fluorescence microscopy (FM), flow cytometry (FC), and Western blotting (WB) techniques. We show that TPGS induces apoptosis in K562 cells through H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> signaling mechanism comprising the activation of a minimal molecular cascade: the kinase JNK&gt;the transcription factor c\u2010JUN&gt;the activation of BCL\u2010only BH3 proapoptotic protein PUMA&gt;loss of mitochondrial membrane potential (<jats:italic>\u0394\u03a8<\/jats:italic><jats:sub>m<\/jats:sub>)&gt;activation of caspase\u20103&gt;chromatin condensation&gt;fragmentation of DNA. Additionally, TPGS oxidizes the stress sensor protein DJ\u20101\u2010Cys106\u2010SH into DJ\u20101\u2010Cys106\u2010SO<jats:sub>3<\/jats:sub> and arrested the cell cycle in the S phase. Remarkably, NAC, SP600125, and NSCSI blocked TPGS\u2010induced OS and apoptosis in K562. Since TPGS is safe in mice and humans, it is especially promising for preclinical and clinical CML leukemia research. Our findings support the view that oxidation therapy offers an important opportunity to eliminate CML.<\/jats:p>","DOI":"10.1155\/2021\/5580288","type":"journal-article","created":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T18:05:28Z","timestamp":1623348328000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Vitamin E TPGS 1000 Induces Apoptosis in the K562 Cell Line: Implications for Chronic Myeloid 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