{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T20:57:20Z","timestamp":1792270640529,"version":"4.4.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,7,9]],"date-time":"2021-07-09T00:00:00Z","timestamp":1625788800000},"content-version":"vor","delay-in-days":189,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Danish Cancer Society, Heinrich Kopp\u2019s Grant"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>DNA released from cells into the peripheral blood is known as cell\u2010free DNA (cfDNA), representing a promising noninvasive source of biomarkers that could be utilized to manage Diffuse Large B\u2010Cell Lymphoma (DLBCL), among other diseases. The procedure for purification and handling of cfDNA is not yet standardized, and various preanalytical variables may affect the yield and analysis of cfDNA, including the purification kits, blood collection tubes, and centrifugation regime. Therefore, we aimed to investigate the impact of these preanalytical variables on the yield of cfDNA by comparing three different purification kits DNeasy Blood &amp; Tissue Kit (Qiagen), QIAamp Circulating Nucleic Acid Kit (Qiagen), and Quick\u2010cfDNA Serum &amp; Plasma Kit (Zymo Research). Two blood collection tubes (BCTs), EDTA\u2010K2 and Cell\u2010Free DNA (Streck), stored at four different time points before plasma was separated and cfDNA purified, were compared, and for EDTA tubes, two centrifugation regimes at 2000 \u00d7 <jats:italic>g<\/jats:italic> and 3000 \u00d7 <jats:italic>g<\/jats:italic> were tested. Additionally, we have tested the utility of long\u2010term archival blood samples from DLBCL patients to detect circulating tumor DNA (ctDNA). We observed a higher cfDNA yield using the QIAamp Circulating Nucleic Acid Kit (Qiagen) purification kit, as well as a higher cfDNA yield when blood samples were collected in EDTA BCTs, with a centrifuge regime at 2000 \u00d7 <jats:italic>g<\/jats:italic>. Moreover, ctDNA detection was feasible from archival plasma samples with a median storage time of nine years.<\/jats:p>","DOI":"10.1155\/2021\/5585148","type":"journal-article","created":{"date-parts":[[2021,7,9]],"date-time":"2021-07-09T18:07:22Z","timestamp":1625854042000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Optimization of Preanalytical Variables for cfDNA Processing and Detection of ctDNA in Archival Plasma Samples"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9550-6156","authenticated-orcid":false,"given":"Marijana","family":"Nesic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie S.","family":"B\u00f8dker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone K.","family":"Terp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2488-435X","authenticated-orcid":false,"given":"Karen","family":"Dybk\u00e6r","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,9]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-01-643569"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-05-577189"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2020.03.015"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0016-8"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-99-112010"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-05-719641"},{"key":"e_1_2_9_7_2","first-page":"241","article-title":"Nuclear acids in human blood plasma","volume":"142","author":"Mandel P.","year":"1948","journal-title":"Comptes rendus des seances de la Societe de biologie et de ses filiales"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0009-8981(01)00665-9"},{"key":"e_1_2_9_9_2","first-page":"1659","article-title":"DNA Fragments in the blood plasma of cancer patients: quantitations and evidence for their origin from apoptotic and necrotic cells","volume":"61","author":"Jahr S.","year":"2001","journal-title":"Cancer research"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.5045\/br.2019.54.2.114"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2013.110"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bdq.2018.12.003"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49860-0"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00002.2014"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2012.45.2011"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3007094"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-635219"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1272-6"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2015.131128"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2017.11.004"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12020312"},{"key":"e_1_2_9_22_2","unstructured":"Cell-Free DNA BCT | Streck 2021 https:\/\/www.streck.com\/products\/stabilization\/cell-free-dna-bct-ivd\/."},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.1184"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.21037\/tlcr.2016.12.03"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2015.07.097"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-018-0382-z"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22670"},{"key":"e_1_2_9_28_2","article-title":"High resolution size analysis of fetal DNA in the urine of pregnant women by paired-end massively parallel sequencing","volume":"7","author":"NBY T.","year":"2012","journal-title":"PLoS One"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2016.05.035"},{"key":"e_1_2_9_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/46.8.1078"},{"key":"e_1_2_9_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2015.02.014"},{"key":"e_1_2_9_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2019.10.005"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2021\/5585148.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2021\/5585148.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2021\/5585148","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T16:07:37Z","timestamp":1735661257000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2021\/5585148"}},"subtitle":[],"editor":[{"given":"Luca","family":"Liberale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1155\/2021\/5585148"],"URL":"https:\/\/doi.org\/10.1155\/2021\/5585148","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"2021-02-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"5585148"}}