{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T07:08:27Z","timestamp":1773644907223,"version":"3.50.1"},"reference-count":13,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T00:00:00Z","timestamp":1619395200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012016","name":"Nipponham Foundation","doi-asserted-by":"publisher","award":["17935758"],"award-info":[{"award-number":["17935758"]}],"id":[{"id":"10.13039\/501100012016","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012016","name":"Nipponham Foundation","doi-asserted-by":"publisher","award":["20H05678"],"award-info":[{"award-number":["20H05678"]}],"id":[{"id":"10.13039\/501100012016","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012016","name":"Nipponham Foundation","doi-asserted-by":"publisher","award":["19K16603"],"award-info":[{"award-number":["19K16603"]}],"id":[{"id":"10.13039\/501100012016","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012016","name":"Nipponham Foundation","doi-asserted-by":"publisher","award":["18K14603"],"award-info":[{"award-number":["18K14603"]}],"id":[{"id":"10.13039\/501100012016","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shimadzu Science Foundation","award":["17935758"],"award-info":[{"award-number":["17935758"]}]},{"name":"Shimadzu Science Foundation","award":["20H05678"],"award-info":[{"award-number":["20H05678"]}]},{"name":"Shimadzu Science Foundation","award":["19K16603"],"award-info":[{"award-number":["19K16603"]}]},{"name":"Shimadzu Science Foundation","award":["18K14603"],"award-info":[{"award-number":["18K14603"]}]},{"DOI":"10.13039\/100015103","name":"Japan Racing Association","doi-asserted-by":"publisher","award":["17935758"],"award-info":[{"award-number":["17935758"]}],"id":[{"id":"10.13039\/100015103","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015103","name":"Japan Racing Association","doi-asserted-by":"publisher","award":["20H05678"],"award-info":[{"award-number":["20H05678"]}],"id":[{"id":"10.13039\/100015103","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015103","name":"Japan Racing Association","doi-asserted-by":"publisher","award":["19K16603"],"award-info":[{"award-number":["19K16603"]}],"id":[{"id":"10.13039\/100015103","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015103","name":"Japan Racing Association","doi-asserted-by":"publisher","award":["18K14603"],"award-info":[{"award-number":["18K14603"]}],"id":[{"id":"10.13039\/100015103","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["17935758"],"award-info":[{"award-number":["17935758"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["20H05678"],"award-info":[{"award-number":["20H05678"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["19K16603"],"award-info":[{"award-number":["19K16603"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["18K14603"],"award-info":[{"award-number":["18K14603"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17935758"],"award-info":[{"award-number":["17935758"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20H05678"],"award-info":[{"award-number":["20H05678"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K16603"],"award-info":[{"award-number":["19K16603"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K14603"],"award-info":[{"award-number":["18K14603"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2021,4,26]]},"abstract":"<jats:p>Tetranor-PGDM is a metabolite of PGD2. Urinary tetranor-PGDM levels were reported to be increased in some diseases, including food allergy, Duchenne muscular dystrophy, and aspirin-intolerant asthma. In this study, we developed a monoclonal antibody (MAb) and a competitive enzyme immunoassay (EIA) for measuring tetranor-PGDM. Spleen cells isolated from mice immunized with tetranor-PGDM were utilized to generate Ab-producing hybridomas. We chose hybridomas and purified MAb against tetranor-PGDM to develop competitive EIA. The assay evaluated the optimal ionic strength, pH, precision, and reliability. Specificity was determined by cross-reactivity to tetranor-PGEM, tetranor-PGFM, and tetranor-PGAM. Recovery was determined by spiking experiments on artificial urine. Optimal ionic strength was 150\u2009mM NaCl, and optimal pH was pH\u20097.5. Metabolites other than tetranor-PGDM did not show any significant cross-reactivity in the EIA. The assay exhibited a half-maximal inhibition concentration (IC50) of 1.79\u2009ng\/mL, limit of detection (LOD) of 0.0498\u2009ng\/mL, and range of quantitation (ROQ) value of 0.252 to 20.2\u2009ng\/mL. The intra- and inter-assay variation for tetranor-PGDM was 3.9\u20136.0% and 5.7\u201310.4%, respectively. The linearity-dilution effect showed excellent linearity under dilution when artificial urine samples were applied to solid-phase extraction (SPE). After SPE, recovery of tetranor-PGDM in artificial urine averaged from 82.3% to 113.5% and was within acceptable limits (80%\u2013120%). We successfully generated one monoclonal antibody and developed a sensitive competitive EIA. The established EIA would be useful for routine detection and monitoring of tetranor-PGDM in research or diagnostic body fluids.<\/jats:p>","DOI":"10.1155\/2021\/5591115","type":"journal-article","created":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T20:21:50Z","timestamp":1619554910000},"page":"1-6","source":"Crossref","is-referenced-by-count":1,"title":["Development of Monoclonal Antibody-Based EIA for Tetranor-PGDM which Reflects PGD2 Production in the Body"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5880-3979","authenticated-orcid":true,"given":"Nanae","family":"Nagata","sequence":"first","affiliation":[{"name":"Department of Animal Radiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4951-1824","authenticated-orcid":true,"given":"Sakura","family":"Masuko","sequence":"additional","affiliation":[{"name":"Department of Animal Radiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rikako","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Animal Radiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3134-0716","authenticated-orcid":true,"given":"Tatsuro","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Animal Radiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7441-7955","authenticated-orcid":true,"given":"Kosuke","family":"Aritake","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Pharmacology, Daiichi University of Pharmacy, 22-1 Tamagawa-cho, Minami-ku, Fukuoka 815-8511, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3328-0796","authenticated-orcid":true,"given":"Takahisa","family":"Murata","sequence":"additional","affiliation":[{"name":"Department of Animal Radiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M706839200"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17798-w"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.06.032"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.braindev.2018.06.012"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.09.019"},{"key":"6","volume-title":"Antibodies : A Laboratory Manual","author":"E. Harlow","year":"1988"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1021\/jf000937j"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/31.8.1264"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1021\/ac0302859"},{"key":"10","first-page":"103","article-title":"New frontiers in agrochemical immunoassay","author":"D. P. Schneider"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/s1570-0232(02)00617-7"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)45802-X"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1941"}],"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2021\/5591115.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2021\/5591115.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2021\/5591115.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T20:21:58Z","timestamp":1619554918000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jir\/2021\/5591115\/"}},"subtitle":[],"editor":[{"given":"Baohui","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,4,26]]},"references-count":13,"alternative-id":["5591115","5591115"],"URL":"https:\/\/doi.org\/10.1155\/2021\/5591115","relation":{},"ISSN":["2314-7156","2314-8861"],"issn-type":[{"value":"2314-7156","type":"electronic"},{"value":"2314-8861","type":"print"}],"subject":[],"published":{"date-parts":[[2021,4,26]]}}}