{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:40:37Z","timestamp":1747190437732,"version":"3.40.5"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,11,9]],"date-time":"2021-11-09T00:00:00Z","timestamp":1636416000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007956","name":"Natural Science Foundation of Nanjing University of Chinese Medicine","doi-asserted-by":"crossref","award":["XZR2020062","CS202030","Cszyy201912"],"award-info":[{"award-number":["XZR2020062","CS202030","Cszyy201912"]}],"id":[{"id":"10.13039\/501100007956","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Changshu Municipal Science and Technology Bureau Supporting Project","award":["XZR2020062","CS202030","Cszyy201912"],"award-info":[{"award-number":["XZR2020062","CS202030","Cszyy201912"]}]},{"name":"Changshu Hospital Affiliated to Nanjing University of Chinese Medicine Youth Fund Supporting Project","award":["XZR2020062","CS202030","Cszyy201912"],"award-info":[{"award-number":["XZR2020062","CS202030","Cszyy201912"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2021,11,9]]},"abstract":"<jats:p>To investigate the effect and mechanism of QingHuaZhiXie prescription on diarrhea predominant irritable bowel syndrome (D-IBS), animal models of rats were used in this study. 48 rats were randomly divided into 6 groups, containing one control group, one animal model group (D-IBS group), and four drug intervention groups (low, medium, and high dosage of QingHuaZhiXie prescription and trimebutine maleate intervention group). Abdominal withdrawal reflex (AWR) and Bristol stool form scale were recorded; the expression levels of inflammatory factors (TNF-\u03b1 and IFN-\u03b3), pathway proteins TLR4, MyD88, NF-\u03baB, and key proteins of tight junction between intestinal epithelial cells (IECs) were detected; the microstructure of intestinal mucosal was observed by hematoxylin and eosin (H&amp;E) staining; MPO activity was detected with immunohistochemical analysis to reflect the inflammation of tissues. Results show that QingHuaZhiXie prescription reduced diarrhea index and intestinal hypersensitivity and intestinal tissue integrity after intervention. MPO activity in QingHuaZhiXie prescription-treated rats was significantly lower relative to their model group. The expression levels of inflammatory factors and TLR4\/MyD88\/NF-\u03baB pathway proteins were repressed, and the protein levels of occludin and claudin-1 increased. Meanwhile, this study also found that the remission effect of QingHuaZhiXie prescription on D-IBS increased with its dosage increase. Hence, as a therapeutic prescription for D-IBS, QingHuaZhiXie prescription could relieve D-IBS symptoms through balancing the inflammatory factors expression by inhibiting the TLR4\/MyD88\/NF-\u03baB pathway and maintaining the function and structure of IECs by improving the protein levels of JAM, occludin, claudin-1, and ZO-1.<\/jats:p>","DOI":"10.1155\/2021\/5792130","type":"journal-article","created":{"date-parts":[[2021,11,9]],"date-time":"2021-11-09T21:20:05Z","timestamp":1636492805000},"page":"1-9","source":"Crossref","is-referenced-by-count":3,"title":["Mechanism of QingHuaZhiXie Prescription Regulating TLR4-IECs Pathway in the Intervention of Diarrhea Predominant Irritable Bowel Syndrome"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6111-8019","authenticated-orcid":true,"given":"Hua","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Anorectal, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu 215500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Anorectal, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu 215500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meijuan","family":"Xi","sequence":"additional","affiliation":[{"name":"Digestive System Department, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Li","sequence":"additional","affiliation":[{"name":"Digestive System Department, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4416-8334","authenticated-orcid":true,"given":"Lijiang","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Anorectal, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu 215500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pop.2017.07.009"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2015.0954"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2019.06.014"},{"issue":"4","key":"4","first-page":"355","article-title":"Updated analysis of irritable bowel syndrome: a review of the literature","volume":"39","author":"M. 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