{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:11:56Z","timestamp":1790687516395,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T00:00:00Z","timestamp":1629244800000},"content-version":"vor","delay-in-days":229,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Scientific and Technological Research Project of Higher Education of Henan Provincial","award":["19A320018"],"award-info":[{"award-number":["19A320018"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>It has been considered that glucose fluctuation (GF) plays a role in renal injury and is related to diabetic nephropathy (DN) development. But the mechanism is still unclear. Aerobic glycolysis has become a topical issue in DN in recent years. There is an internal connection between GF, aerobic glycolysis, and DN. Curcumin (Cur) is a principal curcuminoid of turmeric and possesses specific protective properties in kidney functions. Cur also participates in the regulation of aerobic glycolysis switch. In this study, we first measured the levels of aerobic glycolysis and evaluated Cur\u2019s inhibitory ability in a cell model of HEK\u2010293 under the condition of oscillating high glucose. The results indicated that GF exacerbated inflammation injury, oxidative stress, and apoptosis in HEK\u2010293 cell, while Cur alleviated this cytotoxicity induced by GF. We found that GF increased aerobic glycolysis in HEK\u2010293 cells and Cur presented a dose\u2010dependent weakening effect to this exacerbation. Next, we built a panel of 17 miRNAs and 8 lncRNAs that were previously reported to mediate the Warburg effect. Our RT\u2010qPCR results indicated that GF reduced the miR\u2010489 content in the HEK\u2010293 cell model and Cur could prevent this downregulation. Then, we planned to explore the character of miR\u2010489 in Cur\u2010triggered attenuation of the Warburg effect under GF condition. Our findings presented that Cur prevented GF\u2010triggered aerobic glycolysis by upregulating miR\u2010489 in HEK\u2010293 cells. Next, we choose the miR\u2010489\/LDHA axis for further investigation. We confirmed that Cur prevented GF\u2010triggered aerobic glycolysis via the miR\u2010489\/LDHA axis in HEK\u2010293 cells. In conclusion, this study presented that Cur prevented GF\u2010triggered renal injury by restraining aerobic glycolysis via the miR\u2010489\/LDHA axis in the HEK\u2010293 cell model.<\/jats:p>","DOI":"10.1155\/2021\/6104529","type":"journal-article","created":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T20:05:24Z","timestamp":1629317124000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Curcumin Antagonizes Glucose Fluctuation\u2010Induced Renal Injury by Inhibiting Aerobic Glycolysis via the miR\u2010489\/LDHA Pathway"],"prefix":"10.1155","volume":"2021","author":[{"given":"Xiaomei","family":"Fu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanjie","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhifeng","family":"Mo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyang","family":"Sang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1187-3926","authenticated-orcid":false,"given":"Wenfei","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3013-6833","authenticated-orcid":false,"given":"Wenfeng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,18]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.2174\/1573399817666210309104203"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2019.107843"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0102941"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22503"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2021.11969"},{"key":"e_1_2_9_6_2","first-page":"203","article-title":"Ginkgo biloba attenuates oxidative DNA damage of human umbilical vein endothelial cells induced by intermittent high glucose","volume":"69","author":"He Y. 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