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However, the role of PCA in osteoclasts is not clear. We used RAW264.7 cells (a mouse leukemic monocyte\/macrophage cell line) and bone marrow macrophages (BMMs) to probe the role of PCA in osteoclasts and the underlying mechanism. The effects of PCA on cell activity were evaluated with CCK\u20108 assays. TRAP staining detected mature osteoclasts. Corning Osteo Assay Surface plates were used to examine absorption. The levels of RNA and protein were analyzed, respectively, using RT\u2010PCR and Western blotting. PCA (5\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml) was not toxic to the two cell types but reduced the formation of osteoclasts and bone absorption. Furthermore, PCA restrained the expression of mRNAs encoding proteins associated with osteoclasts and reduced the phosphorylation of proteins in important signaling pathways. The results indicate that PCA inhibits osteoclast differentiation by suppressing NF\u2010<jats:italic>\u03ba<\/jats:italic>B and MAPK activity.<\/jats:p>","DOI":"10.1155\/2021\/6108999","type":"journal-article","created":{"date-parts":[[2021,7,17]],"date-time":"2021-07-17T00:35:09Z","timestamp":1626482109000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["[Retracted] Protocatechualdehyde Inhibits the Osteoclast Differentiation of RAW264.7 and BMM Cells by Regulating NF\u2010\u03baB and MAPK Activity"],"prefix":"10.1155","volume":"2021","author":[{"given":"Yunyun","family":"Qu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Zong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanxin","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2218-4918","authenticated-orcid":false,"given":"Yueran","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7,16]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.2016.1124856"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0178-8"},{"key":"e_1_2_8_3_2","first-page":"1098","article-title":"Bilateral maxillary bisphosphonate-related osteonecrosis of the jaw: a case report","volume":"49","author":"Meng P. 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