{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T19:50:34Z","timestamp":1789674634297,"version":"4.0.1"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T00:00:00Z","timestamp":1635897600000},"content-version":"vor","delay-in-days":306,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170266"],"award-info":[{"award-number":["82170266"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870183"],"award-info":[{"award-number":["81870183"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>This study investigated the effects of chronic intermittent hypoxia (CIH), a model of sleep apnea syndrome (SAS), on cardiac function. SRC\u20103 was extremely lowly expressed in the adult mouse heart tissue, while SRC\u20103 was highly expressed in the adult mouse heart tissue after CIH, suggesting that SRC\u20103 is involved in CIH model. We further studied the role of SRC\u20103 in CIH\u2010induced myocardial injury in mice. Twenty\u2010four healthy Balb\/c male mice (<jats:italic>n<\/jats:italic> = 16, wild type; <jats:italic>n<\/jats:italic> = 8, SRC\u20103 knockout (SRC3\u2010KO)) were randomly divided into three groups: air control (Ctrl), CIH, and CIH+SRC3\u2010KO. Mice were exposed to CIH for 12 weeks. qRT\u2010PCR was used to evaluate cardiac expression of the following genes: 11HSD1, 11HSD2, GR, MR, COX\u20102, OPN, NOX2, HIF\u20101\u2010<jats:italic>\u03b1<\/jats:italic>, IL\u20101<jats:italic>\u03b2<\/jats:italic>, IL\u20106, iNOS, TNF\u2010<jats:italic>\u03b1<\/jats:italic>, PC\u20101, and TGF\u2010<jats:italic>\u03b2<\/jats:italic>. Enzymatic levels of SOD, CAT, MDA, NOS, and NO in the mouse hearts were determined using commercially available kits. Immunohistochemistry (IHC) was used to evaluate NF\u2010<jats:italic>\u03ba<\/jats:italic>B expression in cardiac tissues. A transmission electron microscope (TEM) was used to evaluate myocardial ultrastructure. TUNEL staining was used to assess myocardial cell apoptosis. CIH induced cardiac damage, which was ameliorated in the SRC\u20103 KO mice. CIH significantly increased the heart\u2010to\u2010body weight ratio, expression of all aforementioned genes except 11HSD1, GR, and MR, and increased the levels of MDA, NOS, NO, and NF\u2010<jats:italic>\u03ba<\/jats:italic>B, which were attenuated in the SRC\u20103 KO mice. The CIH group had the lowest SOD and CAT levels, which were partially recovered in the CIH+SRC3\u2010KO group. 11HSD2 gene expression was elevated in both the CIH and CIH+SRC3\u2010KO groups compared to the Ctrl group. The CIH group had severe myocardial cell apoptosis and mitochondrial dysfunction, which were alleviated in the CIH+SRC3\u2010KO group. CIH causes cardiac damage through inducing oxidative stress and inflammation. Knockout of SRC\u20103 ameliorates CIH\u2010induced cardiac damage through antagonizing CIH\u2010triggered molecular changes in cardiac tissue.<\/jats:p>","DOI":"10.1155\/2021\/6372430","type":"journal-article","created":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T22:05:09Z","timestamp":1635977109000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["SRC\u20103 Knockout Attenuates Myocardial Injury Induced by Chronic Intermittent Hypoxia in Mice"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0171-1165","authenticated-orcid":false,"given":"Wanyu","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8595-8829","authenticated-orcid":false,"given":"Hongbo","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4546-9596","authenticated-orcid":false,"given":"Weihua","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9354-7704","authenticated-orcid":false,"given":"Yihua","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6914-3515","authenticated-orcid":false,"given":"Xiangyang","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4709-7909","authenticated-orcid":false,"given":"Wen","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8585-6067","authenticated-orcid":false,"given":"Fang","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9164-4693","authenticated-orcid":false,"given":"Shenhui","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6682-3809","authenticated-orcid":false,"given":"Yonghong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0007-9321","authenticated-orcid":false,"given":"Zhengrong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,3]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9040989"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11325-019-01967-4"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11325-020-02030-3"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11325-012-0669-3"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12872-018-0875-4"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.7555\/JBR.30.20160110"},{"key":"e_1_2_12_7_2","first-page":"93","article-title":"Effect of chronic intermittent hypoxia on hypoxia inducible factor-1alpha in mice","volume":"28","author":"Chen X. 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