{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T20:57:26Z","timestamp":1765486646064,"version":"3.40.5"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T00:00:00Z","timestamp":1628467200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Heilongjiang Provincial Colleges and Universities Basic Research Business Expenses Research Project","award":["2017-QYKYYWF-0761"],"award-info":[{"award-number":["2017-QYKYYWF-0761"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Computational and Mathematical Methods in Medicine"],"published-print":{"date-parts":[[2021,8,9]]},"abstract":"<jats:p>Objective. To explore the protective effects and mechanism of mild hypothermia treatment in the treatment of myocardial ischemia-reperfusion injury. Material and Methods. A total of 20 Sprague-Dawley (SD) rats were assigned to 4 groups: the blank control group, sham operation group, ischemia reperfusion group, and mild hypothermia therapy group (each <jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>n<\/a:mi>\n                        <a:mo>=<\/a:mo>\n                        <a:mn>5<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>). Some indexes were detected. In addition, myocardial cell models of oxygen-glucose deprivation\/reoxygenation injury (OGD) were established. The expression of mRNA IL-6 and TNF-\u03b1 and the key enzyme levels of apoptosis (cleaved-Caspase-3) and the NLRP3 inflammasome\/p53 signaling pathway in the models were determined. Results. The expression of serum IL-6 and TNF-\u03b1 in the mild hypothermia therapy group was significantly lower than that in the ischemia reperfusion group. The mild hypothermia therapy group also showed a significantly lower TUNEL cell count and NLRP3 and p53 phosphorylation levels than the ischemia reperfusion group (all <jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>p<\/c:mi>\n                        <c:mo>&lt;<\/c:mo>\n                        <c:mn>0.05<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula>). The in vitro mild <jats:inline-formula>\n                     <e:math xmlns:e=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\">\n                        <e:mtext>hypothermia<\/e:mtext>\n                        <e:mo>+<\/e:mo>\n                        <e:mtext>OGD<\/e:mtext>\n                     <\/e:math>\n                  <\/jats:inline-formula> group also showed significantly lower mRNA expression of IL-6 and TNF-\u03b1 and levels of cleaved Caspase-3, NLRP3, and phosphorylated p53 protein than the OGD group (all <jats:inline-formula>\n                     <g:math xmlns:g=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\">\n                        <g:mi>p<\/g:mi>\n                        <g:mo>&lt;<\/g:mo>\n                        <g:mn>0.05<\/g:mn>\n                     <\/g:math>\n                  <\/jats:inline-formula>). Conclusion. In conclusion, mild hypothermia therapy can inhibit the apoptosis and myocardial inflammation of cells induced by MI\/R injury in rats and inhibiting the activity of the NLRP3 inflammasome pathway and p53 signaling pathway may be the mechanism.<\/jats:p>","DOI":"10.1155\/2021\/6415275","type":"journal-article","created":{"date-parts":[[2021,8,10]],"date-time":"2021-08-10T20:05:14Z","timestamp":1628625914000},"page":"1-9","source":"Crossref","is-referenced-by-count":7,"title":["Mild Hypothermia Therapy Lowers the Inflammatory Level and Apoptosis Rate of Myocardial Cells of Rats with Myocardial Ischemia-Reperfusion Injury via the NLRP3 Inflammasome Pathway"],"prefix":"10.1155","volume":"2021","author":[{"given":"Renjun","family":"Gao","sequence":"first","affiliation":[{"name":"The Sixth Department of Cardiology, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongye","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Physiology, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Tang","sequence":"additional","affiliation":[{"name":"The Sixth Department of Cardiology, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Cai","sequence":"additional","affiliation":[{"name":"The Sixth Department of Cardiology, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinrui","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Sixth Department of Cardiology, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Zong","sequence":"additional","affiliation":[{"name":"The Sixth Department of Cardiology, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yitong","family":"Li","sequence":"additional","affiliation":[{"name":"The Sixth Department of Cardiology, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5661-4288","authenticated-orcid":true,"given":"Yanli","family":"Wang","sequence":"additional","affiliation":[{"name":"The Sixth Department of Cardiology, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.2147\/iprp.s133088"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-018-0119-4"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1606915"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)30677-8"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1002\/clc.20922"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra071667"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000079019.02601.90"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2014.04.012"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.6515\/ACS.201907_35(4).20190113A"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1136\/heartjnl-2015-308559"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-019-0727-0"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.117.032722"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.4244\/EIJ-D-17-00279"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.cjca.2010.12.027"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.3791\/2464"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.4236\/jbnb.2011.21006"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/j.acvd.2016.05.005"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2006.01292.x"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1097\/01.sla.0000154259.73060.f1"},{"issue":"2","key":"20","first-page":"765","article-title":"Cellular and molecular mechanisms of endothelial ischemia\/reperfusion injury: perspectives and implications for postischemic myocardial protection","volume":"8","author":"Q. 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