{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T01:26:20Z","timestamp":1783041980045,"version":"3.54.6"},"reference-count":21,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T00:00:00Z","timestamp":1617840000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2018YFC1707000"],"award-info":[{"award-number":["2018YFC1707000"]}]},{"name":"National Key Research and Development Program of China","award":["16QNP151"],"award-info":[{"award-number":["16QNP151"]}]},{"name":"National Key Research and Development Program of China","award":["2019MBD-045"],"award-info":[{"award-number":["2019MBD-045"]}]},{"name":"PLA Youth Training Project for Medical Science","award":["2018YFC1707000"],"award-info":[{"award-number":["2018YFC1707000"]}]},{"name":"PLA Youth Training Project for Medical Science","award":["16QNP151"],"award-info":[{"award-number":["16QNP151"]}]},{"name":"PLA Youth Training Project for Medical Science","award":["2019MBD-045"],"award-info":[{"award-number":["2019MBD-045"]}]},{"name":"Medical Big Data and Artificial Intelligence Research and Development Project of PLA General Hospital","award":["2018YFC1707000"],"award-info":[{"award-number":["2018YFC1707000"]}]},{"name":"Medical Big Data and Artificial Intelligence Research and Development Project of PLA General Hospital","award":["16QNP151"],"award-info":[{"award-number":["16QNP151"]}]},{"name":"Medical Big Data and Artificial Intelligence Research and Development Project of PLA General Hospital","award":["2019MBD-045"],"award-info":[{"award-number":["2019MBD-045"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2021,4,8]]},"abstract":"<jats:p>Fuke Qianjin Capsule (FKQJ) is a common TCM compound formula in the treatment of gynecological inflammation-related diseases. This study intends to explore and establish a bioassay method to further improve its quality control. The bioassay method for the determination of anti-inflammatory biopotency was established based on its inhibitory activity on recombinant human cyclooxygenase-2 (COX-2), an active target of FKQJ in the treatment of female pelvic inflammatory disease. We firstly established chemical fingerprint of 20 batches of FKQJ by ultra-high-performance liquid chromatography to identify the components and analyze the chemical similarities. The similarity within different batches of FKQJ was relatively high. The values of similarity of the 19 batches were between 0.973 and 0.995, while one batch\u2019s similarity value was 0.813. Celecoxib, a selective inhibitor of COX-2, was chosen as the positive control drug in COX-2 activity assay to establish an anti-inflammatory biopotency detection method based on parallel line test of qualitative response. The methodological investigation showed that the method possessed good repeatability and precision. Secondly, the anti-inflammatory biopotency of 20 batches of FKQJ for inhibiting COX-2 was determined. The results showed that the biopotency of different batches of FKQJ ranged from 676\u2009U\/\u03bcg to 1310\u2009U\/\u03bcg, with average value of 918\u2009U\/\u03bcg and RSD of 16.7%. Based on multiple linear regression analysis, we found that three contents were highly correlated with the anti-inflammatory biopotency, while chlorogenic acid was validated of the strongest anti-inflammatory activity in vitro. Compared with chemical detection, bioassay can better reflect the quality fluctuation of different batches of products and correlate the known pharmacodynamic targets. The supplement of the bioassay method based on chemical evaluation is helpful to improve the quality control ability of Chinese patent medicine and ensure its clinical efficacy is stable and controllable.<\/jats:p>","DOI":"10.1155\/2021\/6620124","type":"journal-article","created":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T19:25:42Z","timestamp":1617909942000},"page":"1-10","source":"Crossref","is-referenced-by-count":4,"title":["Study on the Bioassay of Anti-Inflammatory Effects of Fuke Qianjin Capsule Based on COX-2 Inhibiting Activity"],"prefix":"10.1155","volume":"2021","author":[{"given":"Run-ran","family":"Ma","sequence":"first","affiliation":[{"name":"College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, Henan 450000, China"},{"name":"China Military Institute of Chinese Medicine, Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-juan","family":"Yang","sequence":"additional","affiliation":[{"name":"Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing 102218, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Huang","sequence":"additional","affiliation":[{"name":"China Military Institute of Chinese Medicine, Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai-shuai","family":"Chen","sequence":"additional","affiliation":[{"name":"China Military Institute of Chinese Medicine, Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-he","family":"Xiao","sequence":"additional","affiliation":[{"name":"China Military Institute of Chinese Medicine, Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8070-9543","authenticated-orcid":true,"given":"Jia-bo","family":"Wang","sequence":"additional","affiliation":[{"name":"China Military Institute of Chinese Medicine, Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China"},{"name":"School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9101-726X","authenticated-orcid":true,"given":"Ming","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Poisoning Treatment, Fifth Medical Center of Chinese PLA General Hospital, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"issue":"7","key":"1","first-page":"55","article-title":"Research progress of Fuke Qianjin capsults (tablets) in gynecological diseases","volume":"15","author":"Y. C. Zhi","year":"2018","journal-title":"China Medical Herald"},{"key":"2","first-page":"58","article-title":"Experimental study on the anti-inflammatory and immune effects of Fuke Qianjin Capsule","volume":"5","author":"H. Tian","year":"2000","journal-title":"Hunan Journal of Traditional Chinese Medicine"},{"key":"3"},{"key":"4","volume-title":"Biological Evaluation of Quality of Traditional Chinese Medicine","author":"X. H. Xiao","year":"2018"},{"issue":"12","key":"5","first-page":"2141","article-title":"Application of an effect-constituents index for the quality evaluation of the traditional Chinese medicine rhubarb","volume":"54","author":"P. Tan","year":"2019","journal-title":"Acta Pharmaceutica Sinica"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2016.00012"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2016.00164"},{"issue":"5","key":"8","first-page":"693","article-title":"Quality evaluation of salvia miltiorrhiza bge. Based on chemical profile and bioassay of anti-platelet aggregation","volume":"45","author":"Z. J. Liu","year":"2017","journal-title":"Chinese Journal of Analytical Chemistry"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-016-1553-7"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2018.11.034"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1186\/0717-6287-47-41"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/s1875-5364(15)30088-1"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2019.104259"},{"issue":"14","key":"14","first-page":"2683","article-title":"Quality evaluation of rhubarb dispensing granules based on multicomponent simultaneous quantitative analysis and bioassay","volume":"42","author":"P. 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Zhao","year":"2020","journal-title":"Modern Chinese Medicine"},{"issue":"8","key":"18","first-page":"601","article-title":"Progress in quality control of moghania","volume":"51","author":"P. Chen","year":"2016","journal-title":"Chinese Pharmaceutical Journal"},{"issue":"9","key":"19","first-page":"122","article-title":"Advances in chemical composition, pharmacological action and quality control of mahonia bealei","volume":"47","author":"L. Hong","year":"2019","journal-title":"Guizhou Agricultural Sciences"},{"issue":"5","key":"20","first-page":"534","article-title":"A study on quality control of Caulis Spatholobi decoction pieces","volume":"33","author":"J. Liu","year":"2019","journal-title":"Chinese Pharmaceutical Affairs"},{"key":"21","first-page":"5938","article-title":"Evolution of the lignin chemical structure during the bioethanol production process and its inhibition to enzymatic hydrolysis","volume-title":"Energy & Fuels","author":"C. Zhao"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2021\/6620124.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2021\/6620124.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2021\/6620124.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T19:26:01Z","timestamp":1617909961000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ecam\/2021\/6620124\/"}},"subtitle":[],"editor":[{"given":"Hua","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,4,8]]},"references-count":21,"alternative-id":["6620124","6620124"],"URL":"https:\/\/doi.org\/10.1155\/2021\/6620124","relation":{},"ISSN":["1741-4288","1741-427X"],"issn-type":[{"value":"1741-4288","type":"electronic"},{"value":"1741-427X","type":"print"}],"subject":[],"published":{"date-parts":[[2021,4,8]]}}}