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This study was designed to investigate the expression variation of IL-10 in the multiple sites including cortex, hippocampus, and lung tissues of neonatal hypoxic-ischemic (HI) rats and explore the crucial role of IL-10 in alleviating HI brain damage. In this study, neonatal Sprague-Dawley rats were subjected to the right common carotid artery ligation, followed by 2\u2009h of hypoxia. The expression of IL-10 in the cortex, hippocampus, and lung tissues was measured with immunohistochemistry, real-time quantitative polymerase chain reaction (RT-qPCR), and western blot (WB). Immunofluorescence double staining was performed to observe the localization of IL-10 in neurons and astrocytes. Moreover, not-targeting and targeting IL-10 siRNA lentivirus vectors were injected into the rats of the negative control (NC) and IL-10 group, respectively, and the mRNA levels of B-cell lymphoma 2 (Bcl-2) and endoplasmic reticulum protein 29 (ERp29) were detected by RT-qPCR following IL-10 silence. The results demonstrated that the IL-10 expression was markedly increased after HI and IL-10 were colocalized with neurons and astrocytes which were badly injured by HI insult. In addition, Bcl-2 and ERp29 were remarkably decreased following IL-10 mRNA interference compared with the NC group. Our findings revealed that IL-10 exerted its antiapoptotic and neuroprotective effects by regulating the expression of Bcl-2 and ERp29, indicating that IL-10 may be a promising molecule target for HIE treatment.<\/jats:p>","DOI":"10.1155\/2021\/6622713","type":"journal-article","created":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T20:43:18Z","timestamp":1622666598000},"page":"1-12","source":"Crossref","is-referenced-by-count":6,"title":["Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2040-7778","authenticated-orcid":true,"given":"Xue","family":"Bai","sequence":"first","affiliation":[{"name":"Department of Cardiac and Cerebral Diseases, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou 646000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1623-5969","authenticated-orcid":true,"given":"Liu-Lin","family":"Xiong","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Medical Sciences, Division of Health Sciences, University of South Australia, Adelaide 5000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4434-0464","authenticated-orcid":true,"given":"Chang-Le","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Cardiac and Cerebral Diseases, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou 646000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao-Li","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5253-7158","authenticated-orcid":true,"given":"Lu-Lu","family":"Xue","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience, Animal Zoology Department, Kunming Medical University, Kunming 650031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Hu","sequence":"additional","affiliation":[{"name":"Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing-Jie","family":"Xia","sequence":"additional","affiliation":[{"name":"Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5678-7437","authenticated-orcid":true,"given":"Jia","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience, Animal Zoology Department, Kunming Medical University, Kunming 650031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8679-1373","authenticated-orcid":true,"given":"Jun-Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiac and Cerebral Diseases, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou 646000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2012-8936","authenticated-orcid":true,"given":"Ting-Hua","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China"},{"name":"Institute of Neuroscience, Animal Zoology Department, Kunming Medical University, Kunming 650031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0135-3970","authenticated-orcid":true,"given":"Si-Jin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Cardiac and Cerebral Diseases, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou 646000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3233\/NRE-2010-0533"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1136\/adc.2008.148205"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1001\/jamapediatrics.2014.3269"},{"key":"4","first-page":"1991","article-title":"Neuroprotective effects of electro acupuncture on hypoxic-ischemic encephalopathy in newborn rats ass","volume":"27","author":"T. 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