{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T01:26:08Z","timestamp":1769217968706,"version":"3.49.0"},"reference-count":71,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,2,4]],"date-time":"2021-02-04T00:00:00Z","timestamp":1612396800000},"content-version":"vor","delay-in-days":34,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Echis carinatus<\/jats:italic> is one of the highly venomous snakes of Pakistan that is responsible for numerous cases of envenomation and deaths. In Pakistan, medicinal plants are commonly used traditionally for snakebite treatment because of their low cost and easy availability in comparison with antivenom. The current research is aimed at evaluating the inhibitory activity of Pakistani medicinal plants against acetylcholinesterase and 5\u2009<jats:sup>\u2032<\/jats:sup>\u2010nucleotidases present in <jats:italic>Echis carinatus<\/jats:italic> venom. Acetylcholinesterase and 5\u2009<jats:sup>\u2032<\/jats:sup>\u2010nucleotidase enzymatic assays were performed at different venom concentrations to check the activity of these enzymes. Methanolic extracts from different parts of plants were used for in vitro determination of their inhibitory activity against 5\u2009<jats:sup>\u2032<\/jats:sup>\u2010nucleotidases in snake venom. Active methanolic extracts were subsequently fractioned using different solvents, and these fractions were also assessed for their anti\u20105\u2009<jats:sup>\u2032<\/jats:sup>\u2010nucleotidase activity. Results of this study exhibited that <jats:italic>Eugenia jambolana<\/jats:italic> Willd. ex O. Berg, <jats:italic>Rubia cordifolia<\/jats:italic> L., <jats:italic>Trichodesma indicum<\/jats:italic> (L.) R. Br., <jats:italic>Calotropis procera<\/jats:italic> (Wild.) R. Br., <jats:italic>Curcuma longa<\/jats:italic> L., and <jats:italic>Fagonia arabica<\/jats:italic> L. were able to significantly (<jats:italic>p<\/jats:italic> &gt; 0.5) neutralize the 5\u2009<jats:sup>\u2032<\/jats:sup>\u2010nucleotidase activity by 88%, 86%, 86%, 85%, 83.7%, and 83%, respectively, compared with a standard antidote (snake venom antiserum). Thus, this study indicates that these plants possess the potential to neutralize one of the toxic enzymatic components of <jats:italic>Echis carinatus<\/jats:italic> venom and hence can help to augment the future efforts of developing alternative therapy for the management of snakebites.<\/jats:p>","DOI":"10.1155\/2021\/6631042","type":"journal-article","created":{"date-parts":[[2021,2,5]],"date-time":"2021-02-05T03:53:22Z","timestamp":1612497202000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Anti\u20105\u2009<sup>\u2032<\/sup>\u2010Nucleotidases (5\u2009<sup>\u2032<\/sup>\u2010ND) and Acetylcholinesterase (AChE) Activities of Medicinal Plants to Combat <i>Echis carinatus<\/i> Venom\u2010Induced Toxicities"],"prefix":"10.1155","volume":"2021","author":[{"given":"Nazia","family":"Aslam","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Syeda","family":"Fatima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia","family":"Khalid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahzad","family":"Hussain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mughal","family":"Qayum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khurram","family":"Afzal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2739-9416","authenticated-orcid":false,"given":"Muhammad Hassham Hassan Bin","family":"Asad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,4]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins10120516"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.63"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.k5317"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000603"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2018.10.016"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2020.03.001"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2014.01.005"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.2462"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2011.03.026"},{"key":"e_1_2_9_10_2","first-page":"37","article-title":"Enzymatic activities of some snake venoms from families Elapidae and Viperidae","volume":"9","author":"Alam J. 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