{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:43:41Z","timestamp":1787165021647,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T00:00:00Z","timestamp":1623369600000},"content-version":"vor","delay-in-days":161,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY21H050006"],"award-info":[{"award-number":["LY21H050006"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>\n                    <jats:italic>Objective<\/jats:italic>\n                    . To observe the protective effect of AC\u2010YVAD\u2010CMK on sepsis\u2010induced acute kidney injury in mice and to explore its possible mechanisms primarily.\n                    <jats:italic>Methods<\/jats:italic>\n                    . Eighteen male C57BL\/6 mice were randomly divided into sham\u2010operated group (Control), cecal ligation and puncture group (CLP), and CLP model treated with AC\u2010YVAD\u2010CMK group (AC\u2010YVAD\u2010CMK) (\n                    <jats:italic>n<\/jats:italic>\n                    = 6 in each group). Mice were sacrificed at 24\u2009h after operation, and blood and kidney tissue samples were collected for analyses. Histologic changes were determined microscopically following HE staining. The expression of Ly\u20106B and CD68 was investigated using immunohistochemistry. Serum concentrations of creatinine (sCR) and blood urea nitrogen (BUN) were measured. Serum levels of interleukin\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    (IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    ), interleukin\u201018 (IL\u201018), TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    , and interleukin\u20106 (IL\u20106) were determined by ELISA. The expressions of Caspas\u20101, NLRP\u20101, IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    , and IL\u201018 in renal tissues were investigated using Western blot. Immunofluorescence staining was used to detect the expression of GSDMD protein in renal tissues.\n                    <jats:italic>Results<\/jats:italic>\n                    . AC\u2010YVAD\u2010CMK treatment significantly alleviates sepsis\u2010induced acute kidney injury, with decreased histological injury in renal tissues, suppresses the accumulation of neutrophils and macrophages in renal tissues, and decreased sCR and BUN level (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05). Attenuation of sepsis\u2010induced acute kidney injury was due to the prohibited production of inflammatory cytokines and decrease expression of Caspas\u20101, NLRP\u20101, IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    , and IL\u201018 in renal tissues. In addition, AC\u2010YVAD\u2010CMK treatment significantly reduced the expression of GSDMD in renal tissues compared to those observed in controls (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05).\n                    <jats:italic>Conclusions<\/jats:italic>\n                    . We demonstrated a marked renoprotective effect of caspase\u20101\u2010inhibitor AC\u2010YVAD\u2010CMK in a rat model of sepsis by inhibition of pyroptosis.\n                  <\/jats:p>","DOI":"10.1155\/2021\/6636621","type":"journal-article","created":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T13:05:45Z","timestamp":1623416745000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Caspase\u20101\u2010Inhibitor AC\u2010YVAD\u2010CMK Inhibits Pyroptosis and Ameliorates Acute Kidney Injury in a Model of Sepsis"],"prefix":"10.1155","volume":"2021","author":[{"given":"Mei","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-tao","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ni-shang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long-jiang","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang-yang","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-ping","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-jie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7965-0425","authenticated-orcid":false,"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,11]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200411253512224"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)62193-5"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.2302\/kjm.48.28"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2015.00262"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1159\/000438843"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026778"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63757-1"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2010.81"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2070"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.4103\/0366-6999.166039"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-018-0894-4"},{"key":"e_1_2_8_12_2","first-page":"5685","article-title":"Inhibition of hepatic cells pyroptosis attenuates CLP-induced acute liver injury","volume":"8","author":"Chen Y. 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