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Its main manifestations are continuous or intermittent burning\u2010like and electroshock\u2010like pain in the affected nerves. Electroacupuncture (EA) is widely used in clinical treatment and exerts effects in alleviating neuropathic pain. In this study, we investigated the effect and underlying mechanism of EA on PHN. Sprague\u2010Dawley rats were treated with resiniferatoxin (RTX) to establish a PHN model and subjected to EA and\/or miR\u2010223\u20103p overexpression (OV) or interference. Mechanical withdrawal latency was measured as an indication of pain sensitivity. Hematoxylin\u2010eosin staining and transmission electron microscopy were performed to observe neuron cell morphology and autophagic vacuoles, respectively. ELISA was performed to detect reactive oxygen species (ROS) production and the levels of tumor necrosis factor\u2010 (TNF\u2010) <jats:italic>\u03b1<\/jats:italic>, inducible nitric oxide synthase (iNOS), interleukin\u2010 (IL\u2010) 6, and IL\u201010. Changes in autophagy and apoptosis\u2010related miRNAs were detected by immunofluorescence and qRT\u2010PCR, respectively. In RTX\u2010treated rats, OV and EA reduced pain sensitivity, decreased the number of eosinophils, and increased that of nerve cells. ROS generation and the levels of TNF\u2010<jats:italic>\u03b1<\/jats:italic> and iNOS were significantly reduced, while those of IL\u20106 and IL\u201010 were increased. OV and EA induced fewer autophagic vacuoles than those in the model group. The expression of autophagy\u2010related protein microtubule\u2010associated protein 1 light chain 3\u2010II, ATG9, and Rab1 was decreased by OV and EA, whereas that of P62 was increased. qRT\u2010PCR revealed that miR\u2010223\u20103p expression in the model group decreased but was increased by EA. EA inhibits neuron cell autophagy in PHN by increasing miR\u2010223\u20103p expression.<\/jats:p>","DOI":"10.1155\/2021\/6637693","type":"journal-article","created":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T21:35:05Z","timestamp":1615239305000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Electroacupuncture Inhibits Autophagy of Neuron Cells in Postherpetic Neuralgia by Increasing the Expression of miR\u2010223\u20103p"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8153-034X","authenticated-orcid":false,"given":"Jing","family":"Zou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyang","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9711-5354","authenticated-orcid":false,"given":"Ning","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,8]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.16917"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2710"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/125094"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2006.09.009"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2013.87"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13148-017-0355-1"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201100209"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2017.00065"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00155"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2016.6044"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2726"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1159\/000455987"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2017.00126"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2017.01.027"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/hct196"},{"key":"e_1_2_9_16_2","first-page":"4","article-title":"Clinical observation on treatment of post-herpetic neuralgia with electroacupuncture at acupoint combined with acupuncture","volume":"1","author":"Sun Y. 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