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Inflammation plays an important role in intervertebral disc degeneration (IDD). The protein follistatin\u2010like 1 (FSTL1) plays a proinflammatory role in a variety of inflammatory diseases. <jats:italic>Objectives<\/jats:italic>. The purpose of this study was to investigate whether IDD could be delayed by inhibiting FSTL\u20101 expression. <jats:italic>Methods<\/jats:italic>. We established a puncture\u2010induced IDD model in wild\u2010type and FSTL\u20101+\/\u2010 mice and collected intervertebral discs (IVDs) from the mice. Safranin O staining was used to detect cartilage loss of IVD tissue, and HE staining was used to detect morphological changes of IVD tissue. We measured the expression of FSTL\u20101 and related inflammatory indicators in IVD tissues by immunohistochemical staining, real\u2010time PCR, and Western blotting. <jats:italic>Results<\/jats:italic>. In the age\u2010induced model of IDD, the level of FSTL\u20101 increased with the exacerbation of degeneration. In the puncture\u2010induced IDD model, FSTL\u20101\u2010knockdown mice showed a reduced degree of degeneration compared with that of wild\u2010type mice. Further experiments showed that FSTL\u20101 knockdown also significantly reduced the level of related inflammatory factors in IVD. In vitro experiments showed that FSTL\u20101 knockdown significantly reduced TNF\u2010<jats:italic>\u03b1<\/jats:italic>\u2010induced inflammation. Specifically, the expression levels of the inflammatory factors COX\u20102, iNOS, MMP\u201013, and ADAMTS\u20105 were reduced. Knockdown of FSTL\u20101 attenuated inflammation by inhibiting the expression of P\u2010Smad1\/5\/8, P\u2010Erk1\/2, and P\u2010P65. <jats:italic>Conclusion<\/jats:italic>. Knockdown of FSTL\u20101 attenuated inflammation by inhibiting the TNF\u2010<jats:italic>\u03b1<\/jats:italic> response and Smad pathway activity and ultimately delayed IDD.<\/jats:p>","DOI":"10.1155\/2021\/6640751","type":"journal-article","created":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T17:35:16Z","timestamp":1618248916000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Follistatin\u2010Like 1 Attenuation Suppresses Intervertebral Disc Degeneration in Mice through Interacting with TNF\u2010<i>\u03b1<\/i> and Smad Signaling 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