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Our previous studies indicated that CD69+CD4+ and CD69+CD8+ T cells in tuberculous pleural effusion (TPE) were antigen-specific memory T cells. However, the phenotypical and functional characteristics of CD8+ TRM cells in tuberculosis remain unknown. We found that CD103+CD8+ T cells were the predominant subset of CD103+ lymphocytes in TPE; both CD103 and CD69 expressed on memory CD8+ T cells from TPE were significantly increased compared with those from paired peripheral blood. Phenotypically, CD103+CD69+ and CD103+CD69-CD8+ T cells expressed higher levels of CD45RO than CD103-CD69+CD8+ T cells did; CD103+CD69-CD8+ T cells highly expressed CD27, CD127, and CD62L and some chemokine receptors. We further compared the functional differences among the four distinct CD45RO+CD8+ T subsets identified by CD103 and CD69 expression. In consist with our published results, CD69+CD8+ T cells, but not CD103+CD8+, produced high levels of IFN-\u03b3 after treatment with BCG in the presence of BFA. Nevertheless, CD103-CD69+ and CD103+CD69+ memory CD8+ T cells expressed higher levels of Granzyme B, while CD103+CD69- memory CD8+ T cells were characterized as a possibly immunosuppressive subset by highly expressing CTLA-4, CD25, and FoxP3. Furthermore, TGF-\u03b2 extremely increased CD103 expression but not CD69 in vitro. Together, CD103+CD8+ T cells form the predominant subset of CD103+ lymphocytes in TPE; CD103 and CD69 expression defines distinct CD8+ TRM-like subsets exhibiting phenotypical and functional heterogeneity. Our findings provide an important theoretical basis to optimize and evaluate new tuberculosis vaccines.<\/jats:p>","DOI":"10.1155\/2021\/6643808","type":"journal-article","created":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T23:38:18Z","timestamp":1619134698000},"page":"1-22","source":"Crossref","is-referenced-by-count":9,"title":["Tissue-Resident Memory-Like CD8+ T Cells Exhibit Heterogeneous Characteristics in Tuberculous Pleural Effusion"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6393-6688","authenticated-orcid":true,"given":"Sifei","family":"Yu","sequence":"first","affiliation":[{"name":"Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, China"},{"name":"Clinical Research Institute, The First People\u2019s Hospital of Foshan, 81 Lingnan Road, Foshan 528000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suihua","family":"Lao","sequence":"additional","affiliation":[{"name":"Chest Hospital of Guangzhou, 62 Hengzhigang Road, Guangzhou 510095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binyan","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2381-4053","authenticated-orcid":true,"given":"Changyou","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, China"},{"name":"Clifford Hospital, Jinan University, No. 3 Hongfu Road, Panyu, Guangzhou 511495, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","unstructured":"WHO publishes global tuberculosis report2020https:\/\/apps.who.int\/iris\/bitstream\/handle\/10665\/336069\/9789240013131-eng.pdf"},{"key":"2","volume-title":"Sustainable development goals"},{"issue":"1","key":"3","first-page":"66","article-title":"Tuberculous pleural effusion","volume":"20","author":"Z. 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