{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T09:17:00Z","timestamp":1759483020438,"version":"3.41.2"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T00:00:00Z","timestamp":1618790400000},"content-version":"vor","delay-in-days":108,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008749","name":"Zhejiang Academy of Agricultural Sciences","doi-asserted-by":"publisher","award":["2010DS700124-ZZ1905"],"award-info":[{"award-number":["2010DS700124-ZZ1905"]}],"id":[{"id":"10.13039\/501100008749","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>Muscovy ducks are among the best meat ducks in the world. The objective of this study was to identify genes related to growth metabolism through transcriptome analysis of the ileal tissue of Muscovy ducks. Duck ileum samples with the highest (H group, <jats:italic>n<\/jats:italic> = 5) and lowest (L group, <jats:italic>n<\/jats:italic> = 5) body weight were selected from two hundred 70\u2010day\u2010old Muscovy ducks for transcriptome analysis by RNA sequencing. In the screening of differentially expressed genes (DEGs) between the H and L groups, a total of 602 DEGs with a fold change no less than 2 were identified, among which 285 were upregulated and 317 were downregulated. Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that glutathione metabolism, pyrimidine metabolism, and protein digestion and absorption processes played a vital role in regulating growth and metabolism. The results showed that 7 genes related to growth and metabolism, namely, <jats:italic>ANPEP<\/jats:italic>, <jats:italic>ENPEP<\/jats:italic>, <jats:italic>UPP1<\/jats:italic>, <jats:italic>SLC2A2<\/jats:italic>, <jats:italic>SLC6A19<\/jats:italic>, <jats:italic>NME4<\/jats:italic>, and <jats:italic>LOC106034733<\/jats:italic>, were significantly expressed in group H, which was consistent with the phenotype results. The validation of these 7 genes using real\u2010time quantitative PCR results indicated that the expression level of <jats:italic>ENPEP<\/jats:italic> was significantly different between the H and L groups (<jats:italic>P<\/jats:italic> &lt; 0.05). This study provides a theoretical basis for exploring the influence of the ileum on growth and metabolism in ducks.<\/jats:p>","DOI":"10.1155\/2021\/6648435","type":"journal-article","created":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T19:27:09Z","timestamp":1618860429000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Transcriptome Analysis Reveals the Genes Involved in Growth and Metabolism in Muscovy 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