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However, high consumption of FA may trigger the transition of precancerous tissue towards malignancy. MicroRNAs (miRNAs) are considered to be potential biomarkers of CRC. Thus, identification of miRNAs of dysregulated genes in CRC cells by detailed analysis of mRNA and miRNA expression profile in the context of FA deficiency could substantially increase our understanding of its oncogenesis. mRNA\u2010seq and miRNA\u2010seq analyses were utilized to investigate the expression of miRNAs in FA\u2010deficient CRC cell line\u2013HCT116 through massive parallel sequencing technology. A total of 38 mRNAs and 168 miRNAs were identified to be differentially expressed between CRC groups with or without FA deficiency. We constructed an miRNA\u2010mRNA network for the vital regulatory miRNAs altered in FA\u2010deficient CRC cells. The mRNAs and miRNAs validated by Western blotting and RT\u2010qPCR were consistent with the sequencing results. Results showed that FA deficiency upregulated some miRNAs thereby inhibiting the expression of critical genes in the endoplasmic reticulum (ER) stress pathway. Dysregulated miRNAs in our miRNA\u2010mRNA network could contribute to CRC cell in response to deficient FA. This work reveals novel molecular targets that are likely to provide therapeutic interventions for CRC.<\/jats:p>","DOI":"10.1155\/2021\/6650181","type":"journal-article","created":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T17:52:31Z","timestamp":1619545951000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["miRNA\u2010mRNA Regulatory Network Reveals miRNAs in HCT116 in Response to Folic Acid Deficiency via Regulating Vital Genes of Endoplasmic Reticulum Stress 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