{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T18:28:31Z","timestamp":1787855311405,"version":"build-2784847793"},"reference-count":56,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,7,27]],"date-time":"2021-07-27T00:00:00Z","timestamp":1627344000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016400","name":"National Research Fund, Kenya","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016400","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Parasitology Research"],"published-print":{"date-parts":[[2021,7,27]]},"abstract":"<jats:p>Introduction. Accurate detection of filarial parasites in humans and vectors is essential for the implementation and evaluation of Global and National Programs to eliminate lymphatic filariasis. Immunological methods to detect infection are available; however, cross-reactivity issues have been reported in most of them. Nucleic acid-based molecular assays offer high levels of specificity and sensitivity and can be used to detect the infections. Methods. In this study, we evaluated loop-mediated isothermal amplification (LAMP) tests to amplify Wuchereria bancrofti DNA in patients\u2019 blood. The amplicons were tested by both pH-sensitive dyes for enhanced visual detection and agarose gel electrophoresis. A closed-tube LAMP assay was also evaluated. Cohen\u2019s Kappa statistics was used for statistical analysis of the assays. 125 patients consented for blood sampling which were used for clinical analysis of LAMP assays with the PCR method used as the \u201cgold standard.\u201d Results. The sensitivity of the evaluated Wuchereria bancrofti LAMP was 92.3%, with a specificity of 97.3% and kappa statistics value of 0.84, which is in a strong agreement. Conclusion. In this study, LAMP assays coupled with fluorescence dye detection have been found to be suitable for diagnosis and monitoring of Wuchereria bancrofti infections in the Kenyan population.<\/jats:p>","DOI":"10.1155\/2021\/6650870","type":"journal-article","created":{"date-parts":[[2021,7,28]],"date-time":"2021-07-28T22:20:11Z","timestamp":1627510811000},"page":"1-9","source":"Crossref","is-referenced-by-count":10,"title":["Optimization of a Loop-Mediated Isothermal Amplification Assay as a Point-of-Care Tool for the Detection of Wuchereria bancrofti in Human Blood in Tana River Delta, Kenya"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8927-4790","authenticated-orcid":true,"given":"Kinyatta","family":"Nancy","sequence":"first","affiliation":[{"name":"Kenya Medical Research Institute, Centre for Biotechnology Research and Development, P.O. 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