{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T19:40:18Z","timestamp":1792698018860,"version":"4.5.0"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T00:00:00Z","timestamp":1618876800000},"content-version":"vor","delay-in-days":109,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"\n Background<\/jats:italic>\n . The leaves of\n Hagenia abyssinica<\/jats:italic>\n have been used in the management of diabetes mellitus in Ethiopian folk medicine. Thus, this study is aimed at investigating the\n in vitro \u03b1<\/jats:italic>\n \u2010amylase and\n \u03b1<\/jats:italic>\n \u2010glucosidase inhibitory and antioxidant activities of the crude extract and solvent fractions of\n H. abyssinica<\/jats:italic>\n leaves.\n Methods<\/jats:italic>\n . The\n in vitro \u03b1<\/jats:italic>\n \u2010amylase and\n \u03b1<\/jats:italic>\n \u2010glucosidase inhibitory and antioxidant activities of the plant extract were assessed using 3,5\u2010dinitrosalicylic acid (DNSA), p\u2010nitro\u2010phenyl\u2010a\u2010D glucopyranoside (p\u2010NPG), and 1,1\u2010diphenyl\u20102\u2010picrylhydrazyl (DPPH) assays, respectively. Each value of percent inhibition of\n \u03b1<\/jats:italic>\n \u2010amylase,\n \u03b1<\/jats:italic>\n \u2010glucosidase, and DPPH scavenging effect was presented as means \u00b1 SEM (\n n<\/jats:italic>\n = 3).\n Results<\/jats:italic>\n . The\n \u03b1<\/jats:italic>\n \u2010amylase inhibitory activity of the crude extract and solvent fractions was found to be concentration\u2010dependent. The strongest activity was exhibited by the crude extract at the highest concentration with a percentage inhibition of 74.52% (IC\n 50<\/jats:sub>\n , 14.52\u2009\n \u03bc<\/jats:italic>\n g\/ml) followed by water fraction 68.24% (IC\n 50<\/jats:sub>\n , 16.31\u2009\n \u03bc<\/jats:italic>\n g\/ml), ethyl acetate fraction 61.57% (IC\n 50<\/jats:sub>\n , 18.73\u2009\n \u03bc<\/jats:italic>\n g\/ml), and chloroform fraction 56.87% (IC\n 50<\/jats:sub>\n , 21.57\u2009\n \u03bc<\/jats:italic>\n g\/ml) of\n H. abyssinica<\/jats:italic>\n leaves. In the\n \u03b1<\/jats:italic>\n \u2010glucosidase inhibition assay, the maximum activity was exhibited by the\n aqueous<\/jats:italic>\n fraction 62.54% (IC\n 50<\/jats:sub>\n , 11.67\u2009\n \u03bc<\/jats:italic>\n g\/ml) followed by ethyl acetate fraction 54.97% (IC\n 50<\/jats:sub>\n , 15.89\u2009\n \u03bc<\/jats:italic>\n g\/ml), crude extract 46.79% (IC\n 50<\/jats:sub>\n , >16.5\u2009\n \u03bc<\/jats:italic>\n g\/ml), and chloroform fraction 36.44% (IC\n 50<\/jats:sub>\n , >16.5\u2009\n \u03bc<\/jats:italic>\n g\/ml). In the antioxidant assay, the crude extract exhibited the highest antioxidant activity 86.36% (IC\n 50<\/jats:sub>\n , 10.25\u2009\n \u03bc<\/jats:italic>\n g\/ml) followed by water fraction 78.59% (IC\n 50<\/jats:sub>\n , 13.86\u2009\n \u03bc<\/jats:italic>\n g\/ml), ethyl acetate fraction 71.58% (IC\n 50<\/jats:sub>\n , 16.34\u2009\n \u03bc<\/jats:italic>\n g\/ml), and chloroform fraction 63.65% (IC\n 50<\/jats:sub>\n , 18.83\u2009\n \u03bc<\/jats:italic>\n g\/ml).\n Conclusion<\/jats:italic>\n . This study has revealed that\n H. abyssinica<\/jats:italic>\n leaves possess noticeable\n in vitro \u03b1<\/jats:italic>\n \u2010amylase and\n \u03b1<\/jats:italic>\n \u2010glucosidase inhibitory and antioxidant activities.\n <\/jats:p>","DOI":"10.1155\/2021\/6652777","type":"journal-article","created":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T21:41:25Z","timestamp":1618954885000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["In Vitro \u03b1<\/i>\n \u2010Amylase and\n \u03b1<\/i>\n \u2010Glucosidase Inhibitory and Antioxidant Activities of the Crude Extract and Solvent Fractions of\n Hagenia abyssinica<\/i>\n Leaves"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7030-2782","authenticated-orcid":false,"given":"Zemene Demelash","family":"Kifle","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simachew Gidey","family":"Debeb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5564-7176","authenticated-orcid":false,"given":"Yaschilal Muche","family":"Belayneh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,20]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/ijosup.2016.1"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1586\/17512433.2016.1100072"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2020.558641"},{"key":"e_1_2_12_4_2","volume-title":"Modul Pendidikan dan Pelatihan Prajabatan Golongan III. 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