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By recruiting Th2 cells in the dermis, CCL17 and CCL22 promote the development of inflammation in atopic skin. The aim of this research was to develop a plant extract whose biological properties, when applied topically, could be beneficial for people with atopic\u2010prone skin. The strategy which was followed consisted in identifying ligands able to neutralize the biological activity of CCL17 and CCL22. Thus, an <jats:italic>in silico<\/jats:italic> molecular modeling and a generic screening assay were developed to screen natural molecules binding and blocking these two chemokines. <jats:italic>N<\/jats:italic>\u2010Feruloylserotonin was identified as a neutraligand of CCL22 in these experiments. A cornflower extract containing <jats:italic>N<\/jats:italic>\u2010feruloylserotonin was selected for further <jats:italic>in vitro<\/jats:italic> tests: the gene expression modulation of inflammation biomarkers induced by CCL17 or CCL22 in the presence or absence of this extract was assessed in the HaCaT keratinocyte cell line. Additionally, the same cornflower extract in another vehicle was evaluated in parallel with <jats:italic>N<\/jats:italic>\u2010feruloylserotonin for cyclooxygenase\u20102 (COX\u20102) and 5\u2010lipoxygenase (5\u2010LOX) enzymatic cellular inhibition. The cornflower extract was shown to neutralize the two chemokines <jats:italic>in vitro<\/jats:italic>, inhibited COX\u20102 and 5\u2010LOX, and demonstrated anti\u2010inflammatory activities due mainly to the presence of <jats:italic>N<\/jats:italic>\u2010feruloylserotonin. Although these findings would need to be confirmed in an <jats:italic>in vivo<\/jats:italic> study, the <jats:italic>in vitro<\/jats:italic> studies lay the foundation to explain the benefits of the cornflower extract when applied topically to individuals with atopic\u2010prone skin.<\/jats:p>","DOI":"10.1155\/2021\/6652791","type":"journal-article","created":{"date-parts":[[2021,9,13]],"date-time":"2021-09-13T15:51:00Z","timestamp":1631548260000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["A Cornflower Extract Containing <i>N<\/i>\u2010Feruloylserotonin Reduces Inflammation in Human Skin by Neutralizing CCL17 and CCL22 and Inhibiting COX\u20102 and 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