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In this study, a trial of imaging CD8 with our newly developed sequential multiple\u2010agent receptor targeting (SMART) technology was conducted. Mice bearing a subcutaneous colorectal CT26 tumor received three times different immunotherapy treatments (PD1 or CTLA4 or combined). On either day 7 or day 14 after the first time treatment, the PET imaging study was performed with sequentially administered TCO\u2010modified anti\u2010CD8 antibody and <jats:sup>64<\/jats:sup>Cu\u2010labeled MeTz\u2010NOTA\u2010RGD. However, no positive response was detected, probably due to (1) inappropriate selection of biomarkers for the SMART strategy, (2) limited TCO modification on the anti\u2010CD8 antibody, and (3) inadequate response of the CT26 tumor to the selected immunotherapies. Therefore, the potential of applying SMART in imaging CD8 was not demonstrated in this study, and further optimization will be necessary before it can be applied in imaging CD8.<\/jats:p>","DOI":"10.1155\/2021\/6654262","type":"journal-article","created":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T18:05:59Z","timestamp":1623348359000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["PET Imaging of CD8 via SMART for Monitoring the Immunotherapy 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