{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T02:08:31Z","timestamp":1761962911718,"version":"3.41.2"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T00:00:00Z","timestamp":1624492800000},"content-version":"vor","delay-in-days":174,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Purpose<\/jats:italic>. The aim of this work was to analyze the complete genome of probiotic bacteria <jats:italic>Lactobacillus plantarum<\/jats:italic> 8 RA 3, <jats:italic>Lactobacillus fermentum<\/jats:italic> 90 TC\u20104, <jats:italic>Lactobacillus fermentum<\/jats:italic> 39, <jats:italic>Bifidobacterium bifidum<\/jats:italic> 791, <jats:italic>Bifidobacterium bifidum<\/jats:italic> 1, and <jats:italic>Bifidobacterium longum<\/jats:italic> 379 and to test their activity against influenza A and SARS\u2010CoV\u20102 viruses. <jats:italic>Methods<\/jats:italic>. To confirm the taxonomic affiliation of the bacterial strains, MALDI TOF mass spectrometry and biochemical test systems were used. Whole genome sequencing was performed on the Illumina Inc. MiSeq platform. To determine the antiviral activity, A\/Lipetsk\/1V\/2018 (H1N1 pdm09) (EPI_ISL_332798) and A\/common gull\/Saratov\/1676\/2018 (H5N6) (EPI_ISL_336925) influenza viruses and SARS\u2010CoV\u20102 virus strain Australia\/VIC01\/2020 (GenBank: MT007544.1) were used. <jats:italic>Results<\/jats:italic>. All studied probiotic bacteria are nonpathogenic for humans and do not contain the determinants of transmission\u2010type antibiotic resistance and integrated plasmids. Resistance to antibiotics of different classes is explained by the presence of molecular efflux pumps of the MatE and MFS families. Cultures of <jats:italic>L. fermentum<\/jats:italic> 90 TC 4, <jats:italic>L<\/jats:italic>. <jats:italic>plantarum<\/jats:italic> 8 RA 3, and <jats:italic>B. bifidum<\/jats:italic> 791 showed a pronounced activity against influenza A viruses in MDCK cells. Activity against the SARS\u2010CoV\u20102 virus was demonstrated only by the <jats:italic>L. fermentum<\/jats:italic> 90 TC 4 strain in VERO cells. <jats:italic>Conclusions<\/jats:italic>. The studied probiotic bacteria are safe, have antiviral activity, and are of great importance for the prevention of diseases caused by respiratory viruses that can also infect the human intestine.<\/jats:p>","DOI":"10.1155\/2021\/6662027","type":"journal-article","created":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T15:50:06Z","timestamp":1624549806000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Genome Features and <i>In Vitro<\/i> Activity against Influenza A and SARS\u2010CoV\u20102 Viruses of Six Probiotic Strains"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3136-9500","authenticated-orcid":false,"given":"Irina V.","family":"Soloveva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2354-7688","authenticated-orcid":false,"given":"Tatyana N.","family":"Ilyicheva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1339-6732","authenticated-orcid":false,"given":"Vasiliy Yu.","family":"Marchenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3340-8750","authenticated-orcid":false,"given":"Oleg V.","family":"Pyankov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7753-5730","authenticated-orcid":false,"given":"Anna G.","family":"Tochilina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3402-1160","authenticated-orcid":false,"given":"Irina V.","family":"Belova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1162-2417","authenticated-orcid":false,"given":"Vladimir A.","family":"Zhirnov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolay I.","family":"Bormotov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maksim O.","family":"Skarnovich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5634-1785","authenticated-orcid":false,"given":"Aleksander G.","family":"Durymanov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4750-5925","authenticated-orcid":false,"given":"Svetlana B.","family":"Molodtsova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekaterina I.","family":"Filippova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alena S.","family":"Ovchinnikova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia V.","family":"Magerramova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7009-0748","authenticated-orcid":false,"given":"Alexander B.","family":"Ryzhikov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1314-281X","authenticated-orcid":false,"given":"Rinat A.","family":"Maksyutov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,6,24]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-017-8182-z"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.22587\/aeb.2018.12.1.4"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-8244(03)00147-0"},{"volume-title":"Lactobacillus, molecular biology. From genomics to probiotics","year":"2009","author":"Ljungh A.","key":"e_1_2_9_4_2"},{"volume-title":"Bifidobacteria: genomics and molecular aspects","year":"2010","author":"Mayo B.","key":"e_1_2_9_5_2"},{"volume-title":"The human microbiota and chronic disease: dysbiosis as a cause of human pathology","year":"2016","author":"Henderson B.","key":"e_1_2_9_6_2"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-014-9162-6"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053043"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.07.064"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.04.027"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.36233\/0372-9311-2016-2-16-23"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1134\/S1022795416090039"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077302"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm360"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03015-13"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"e_1_2_9_18_2","unstructured":"April 2020 http:\/\/www.who.int\/influenza\/human_animal_interface\/H5N1_cumulative_table_archives\/en\/."},{"key":"e_1_2_9_19_2","unstructured":"April 2020 http:\/\/www.fao.org\/ag\/againfo\/programmes\/en\/empres\/H7N9\/situation_update.html."},{"key":"e_1_2_9_20_2","unstructured":"World Health Organization WHO Director-General\u2019s opening remarks at the media briefing on COVID-19 \u2013 11 March April 2020 https:\/\/www.who.int\/dg\/speeches\/detail\/who-director-general-s-opening-remarks-at-the-media-briefing-on-covid-19---11-march-2020."},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2020.102463"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1729071"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30183-5"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2017.03.010"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-9403-9"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-015-5302-2"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-016-0567-6"},{"key":"e_1_2_9_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2006.10.044"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01699"},{"key":"e_1_2_9_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12223-018-0648-6"},{"key":"e_1_2_9_31_2","doi-asserted-by":"publisher","DOI":"10.1079\/bjn19980108"},{"key":"e_1_2_9_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/jam.13069"},{"key":"e_1_2_9_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12560-013-9135-z"},{"key":"e_1_2_9_34_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2017-14151"},{"key":"e_1_2_9_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059892"},{"key":"e_1_2_9_36_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13030379"},{"key":"e_1_2_9_37_2","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20203638"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2021\/6662027.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2021\/6662027.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2021\/6662027","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T14:16:32Z","timestamp":1735654592000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2021\/6662027"}},"subtitle":[],"editor":[{"given":"San-Ji","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1155\/2021\/6662027"],"URL":"https:\/\/doi.org\/10.1155\/2021\/6662027","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"type":"print","value":"2314-6133"},{"type":"electronic","value":"2314-6141"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"2020-10-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"6662027"}}