{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:48:48Z","timestamp":1787017728633,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T00:00:00Z","timestamp":1617926400000},"content-version":"vor","delay-in-days":98,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81774419"],"award-info":[{"award-number":["81774419"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>\n                    <jats:italic>Purpose<\/jats:italic>\n                    . We tried to investigate whether electroacupuncture (EA) can reduce inflammation of dry eye disease (DED) by regulating\n                    <jats:italic>\u03b1<\/jats:italic>\n                    7nAChR and inhibiting the NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B signaling pathway.\n                    <jats:italic>Methods<\/jats:italic>\n                    . Healthy New Zealand white rabbits were treated with scopolamine hydrobromide (Scop) for 21 consecutive days to establish the DED animal model. After 21 days, EA, fluorometholone (Flu), and\n                    <jats:italic>\u03b1<\/jats:italic>\n                    7nAChR antagonist (\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010BGT) treatments were performed, and the Scop injection was continued until day 35. During treatment, the fluorescence staining of the corneal epithelium and the level of tear flow were observed. The influence of EA on the LG pathology and inflammatory factors ACh,\n                    <jats:italic>\u03b1<\/jats:italic>\n                    7nAChR, and NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B was detected using the LG histopathology, transmission electron microscopy (TEM), cytokine protein chip technology, enzyme\u2010linked immunosorbent assay (ELISA), and Western blot.\n                    <jats:italic>Results<\/jats:italic>\n                    . The EA stimulation can reduce the corneal epithelial damage and repair epithelial cell ultrastructure, promote the tear secretion, relieve the LG atrophy and decrease lipid droplet accumulation in LG acinar cell, and reduce the levels of inflammatory cytokines (i.e., IL\u20101, MIP\u20101b, TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    , and IL\u20108) in the LG. The protective effect of EA on the inflammation and the ocular surface is similar to the corticosteroid Flu. EA and Flu can upregulate the expression of the\n                    <jats:italic>\u03b1<\/jats:italic>\n                    7nAChR and downregulate the expression of NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B. The\n                    <jats:italic>\u03b1<\/jats:italic>\n                    7nAChR antagonist\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010BGT can reverse the protective effect of EA on the LG and the inhibitory effect on the NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B pathway and the expression of inflammatory factors but cannot affect the expression of Flu on the NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B pathway and inflammatory factors.\n                    <jats:italic>Conclusion<\/jats:italic>\n                    . These results prove that EA can alleviate DEDs by stimulating the acupoints around the eyes. These beneficial effects are related to the upregulation of\n                    <jats:italic>\u03b1<\/jats:italic>\n                    7nAChR and the downregulation of NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B in the LG. The protective effect of LG is mediated through the anti\u2010inflammatory pathway mediated by\n                    <jats:italic>\u03b1<\/jats:italic>\n                    7nAChR. EA can reduce the NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B P65 nuclear transcription and reduce inflammatory factors by regulating\n                    <jats:italic>\u03b1<\/jats:italic>\n                    7nAChR. This expression indicates that the\n                    <jats:italic>\u03b1<\/jats:italic>\n                    7nAChR\/NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B signaling pathway may serve as a potential therapeutic target for EA to treat DEDs.\n                  <\/jats:p>","DOI":"10.1155\/2021\/6673610","type":"journal-article","created":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T20:35:06Z","timestamp":1618000506000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Electroacupuncture Alleviates Inflammation of Dry Eye Diseases by Regulating the\n                    <i>\u03b1<\/i>\n                    7nAChR\/NF\u2010\n                    <i>\u03ba<\/i>\n                    B Signaling 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