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Fifteen participants were enrolled, including five patients with DM complicated with ILDs prior to treatment with circulating anti\u2010MDA5 antibody\u2010positive status [DM\u2010ILD\u2010MDA5 Ab(+)], five DM patients without ILDs who were negative for 16 detectable myositis\u2010specific antibodies [DM\u2010nonILD\u2010MSA16(\u2010)], and five age\u2010 and gender\u2010matched healthy donor controls (HCs). The characteristics of the exosomes extracted by Ribo\u2122 Exosome Isolation Reagent were identified using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and flow cytometry. Differentially expressed miRNAs, determined by next\u2010generation deep sequencing, were identified through the criteria of \u2223log2\u2009fold\u2009change | \u22651 and <jats:italic>P<\/jats:italic> &lt; 0.01. A total of 38 miRNAs were significantly upregulated in exosomes from patients with DM\u2010ILD\u2010MDA5 Ab(+) compared to those from HC, while 21 miRNAs were significantly downregulated. Compared to exosomes derived from patients with DM\u2010nonILD\u2010MSA16(\u2010), 51 miRNAs were significantly upregulated and 33 miRNAs were significantly downregulated from patients with DM\u2010ILD\u2010MDA5 Ab(+). A total of 73 exosomal miRNAs were significantly differentially expressed between DM\u2010nonILD\u2010MSA16(\u2010) and HC. In particular, two miRNAs, <jats:italic>Homo sapiens<\/jats:italic>\u2010 (hsa\u2010) miR\u20104488 and hsa\u2010miR\u20101228\u20105p, were common differentially expressed miRNAs among three comparisons. GO and KEGG analyses suggested that several pathways may contribute the pathogenesis of DM\u2010ILD\u2010MDA5 Ab(+) and DM\u2010nonILD\u2010MSA16(\u2010), while PPI network analysis of hsa\u2010miR\u20104488 and hsa\u2010miR\u20101228\u20105p indicated that their predicted target genes, DExD\u2010box helicase 39B and MDM2, may be involved in the mechanisms of DM\u2010ILD\u2010MDA5 Ab(+).<\/jats:p>","DOI":"10.1155\/2021\/6676107","type":"journal-article","created":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T19:22:03Z","timestamp":1627586523000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Plasma\u2010Derived Exosomal hsa\u2010miR\u20104488 and hsa\u2010miR\u20101228\u20105p: Novel Biomarkers for Dermatomyositis\u2010Associated Interstitial Lung Disease with Anti\u2010Melanoma Differentiation\u2010Associated Protein 5 Antibody\u2010Positive Subset"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4084-4067","authenticated-orcid":false,"given":"Danli","family":"Zhong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanyuan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4541-9898","authenticated-orcid":false,"given":"Qian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,29]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules19021568"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v3.23743"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2018.41"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2018.48"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2018.56"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.21023"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.21023"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kep375"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2014.12.012"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2017.12.010"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00296-011-2323-y"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.160682"},{"key":"e_1_2_12_14_2","first-page":"891","article-title":"Prevalence and clinical significance of anti-MDA5 antibodies in European patients with polymyositis\/dermatomyositis","volume":"32","author":"Ceribelli A.","year":"2014","journal-title":"Clinical and Experimental Rheumatology"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-015-2935-9"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197502132920706"},{"key":"e_1_2_12_17_2","doi-asserted-by":"crossref","unstructured":"R\u00f6nnelidJ. 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