{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:32:36Z","timestamp":1770748356984,"version":"3.49.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,6,8]],"date-time":"2021-06-08T00:00:00Z","timestamp":1623110400000},"content-version":"vor","delay-in-days":158,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003621","name":"Ministry of Science, ICT and Future Planning","doi-asserted-by":"publisher","award":["2018R1A2A3075425"],"award-info":[{"award-number":["2018R1A2A3075425"]}],"id":[{"id":"10.13039\/501100003621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>Protease\u2010activated receptor 2 (PAR2) is a member of G\u2010protein\u2010coupled receptors and affects ligand\u2010modulated calcium signaling. Although PAR2 signaling promotes obesity and adipose tissue inflammation in high fat\u2010 (HF\u2010) fed conditions, its role in adipocyte differentiation under nonobesogenic conditions needs to be elucidated. Here, we used several tissues and primary\u2010cultured adipocytes of mice lacking PAR2 to study its role in the development of adipose tissues. C57BL\/6J mice with PAR2 deficiency exhibited a mild lipodystrophy\u2010like phenotype in a chow diet\u2010fed condition. When adipocyte differentiation was examined using primary\u2010cultured preadipocytes, PAR2 deficiency led to a notable decrease in adipocyte differentiation and related protein expression, and PAR2 agonist treatment elevated adipocyte differentiation. Regarding the mechanism, PAR2\u2010deficient preadipocytes exhibited impaired mitochondrial energy consumption. Further studies indicated that calcium\u2010related signaling pathways for mitochondrial biogenesis are disrupted in the adipose tissues of PAR2\u2010deficient mice and PAR2\u2010deficient preadipocytes. Also, a PAR2 antagonist elevated mitochondrial reactive oxygen species and reduced the MitoTracker fluorescent signal in preadipocytes. Our studies revealed that PAR2 is important for the development of adipose tissue under basal conditions through the regulation of mitochondrial biogenesis and adipocyte differentiation.<\/jats:p>","DOI":"10.1155\/2021\/6683033","type":"journal-article","created":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T06:36:30Z","timestamp":1623220590000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["PAR2 Deficiency Induces Mitochondrial ROS Generation and Dysfunctions, Leading to the Inhibition of Adipocyte Differentiation"],"prefix":"10.1155","volume":"2021","author":[{"given":"Yeo Jin","family":"Park","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3265-1310","authenticated-orcid":false,"given":"Bonggi","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dae 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