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However, the mechanism by which MSCs affect the progression of liver fibrosis by regulating miRNA expression remains unclear. Here, we investigated whether human umbilical cord MSCs (HUC\u2010MSCs) attenuated hepatic fibrosis by regulating miR\u2010455\u20103p and its target gene. Significantly upregulated miRNA (miR\u2010455\u20103p) was screened out by GEO datasets analysis and coculture HUC\u2010MSCs with hepatic stellate cell (HSC) LX\u20102 cells. p21\u2010activated kinase\u20102 (PAK2) was forecasted to be the target gene of miR\u2010455\u20103p by bioinformatics analyses and confirmed by luciferase reporter assay. HUC\u2010MSCs were transplanted into mice with carbon tetrachloride\u2010 (CCl<jats:sub>4<\/jats:sub>\u2010) induced liver fibrosis, the result showed that HUC\u2010MSC transplantation significantly ameliorated the severity of CCl<jats:sub>4<\/jats:sub>\u2010induced liver fibrosis, attenuated collagen deposition, improved liver function by reducing the expression of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, upregulated miR\u2010455\u20103p, and suppressed PAK2 expression of liver tissue in mice. Taken together, our study suggests that HUC\u2010MSCs inhibit the activation of HSCs and mouse CCl<jats:sub>4<\/jats:sub>\u2010induced liver fibrosis by upregulation of miR\u2010455\u20103p through targeting PAK2.<\/jats:p>","DOI":"10.1155\/2021\/6685605","type":"journal-article","created":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T02:05:14Z","timestamp":1614305114000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Human Umbilical Cord Mesenchymal Stem Cells Ameliorate Hepatic Stellate Cell Activation and Liver Fibrosis by Upregulating MicroRNA\u2010455\u20103p through Suppression of p21\u2010Activated 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