{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T08:14:37Z","timestamp":1767168877937,"version":"build-2238731810"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T00:00:00Z","timestamp":1617840000000},"content-version":"vor","delay-in-days":97,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>\n                    <jats:italic>Objectives<\/jats:italic>\n                    . To investigate the roles of miR\u2010221 in spinal cord injury (SCI) as well as the underlying mechanism.\n                    <jats:italic>Methods<\/jats:italic>\n                    . A mouse model of SCI was generated and used to examine dynamic changes in grip strength of the mouse upper and lower limbs. The expression of miR\u2010221 and tumor necrosis factor\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    (TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    ) was detected by RT\u2010qPCR and Western blot. Levels of inflammation and oxidative stress in microglia cells of the injured mice overexpressing miR\u2010221 were then measured by ELISA. Bioinformatics analysis and dual\u2010luciferase reporter assay were conducted to identify the miR\u2010221 target.\n                    <jats:italic>Results<\/jats:italic>\n                    . We successfully constructed SCI mouse model. The results of qRT\u2010PCR showed that miR\u2010221 was gradually upregulated in the spinal cord tissue of mice in the SCI group with the prolonged injury time. At the same time, the mRNA and protein of TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    gradually decreased. We further confirmed through cell experiments that the inflammatory factors TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    and IL\u20106, as well as iNOS and eROS, were upregulated in spinal cord microglia cells of SCI mice, and upregulation of miR\u2010122 can inhibit their expression. Finally, the luciferase reporter experiment confirmed that miR\u2010122 targeted TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    .\n                    <jats:italic>Conclusions<\/jats:italic>\n                    . We present evidence that miR\u2010221 promotes functional recovery of the injured spinal cord through targeting TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    , while alleviating inflammatory response and oxidative stress.\n                  <\/jats:p>","DOI":"10.1155\/2021\/6687963","type":"journal-article","created":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T19:59:41Z","timestamp":1617911981000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["[Retracted] miRNA\u2010221 Regulates Spinal Cord Injury\u2010Induced Inflammatory Response through Targeting TNF\u2010\u03b1 Expression"],"prefix":"10.1155","volume":"2021","author":[{"given":"Feng","family":"Sun","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiwei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianwen","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhui","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianli","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5954-4454","authenticated-orcid":false,"given":"Yansong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,4,8]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-018-0597-2"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.aco.0000162838.56344.88"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1364\/AO.52.000110"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2016.4915"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.4103\/2152-7806.83732"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1159\/000495717"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_201804_14709"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-017-0858-y"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2296"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.9058"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.156983"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00168.2010"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034534"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2014.00053"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/2398521"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2009.05.003"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2011.00146"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.08.008"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1519207"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1159\/000494165"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.7816"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20170755"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.07.162"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.3791\/3313"}],"updated-by":[{"DOI":"10.1155\/2024\/9842930","type":"retraction","label":"Retraction","source":"retraction-watch","updated":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T00:00:00Z","timestamp":1704758400000},"record-id":"57624"},{"DOI":"10.1155\/2024\/9842930","type":"retraction","label":"Retraction","source":"publisher","updated":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T00:00:00Z","timestamp":1704758400000}}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2021\/6687963.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2021\/6687963.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2021\/6687963","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T01:52:04Z","timestamp":1735609924000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2021\/6687963"}},"subtitle":[],"editor":[{"given":"Zhenbo","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1155\/2021\/6687963"],"URL":"https:\/\/doi.org\/10.1155\/2021\/6687963","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"2020-11-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"6687963"}}