{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:21:45Z","timestamp":1792639305393,"version":"4.5.0"},"reference-count":20,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,1,5]],"date-time":"2021-01-05T00:00:00Z","timestamp":1609804800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008402","name":"Chengdu University of Traditional Chinese Medicine","doi-asserted-by":"publisher","award":["2020JCRC015"],"award-info":[{"award-number":["2020JCRC015"]}],"id":[{"id":"10.13039\/501100008402","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008402","name":"Chengdu University of Traditional Chinese Medicine","doi-asserted-by":"publisher","award":["2020XSGG024"],"award-info":[{"award-number":["2020XSGG024"]}],"id":[{"id":"10.13039\/501100008402","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008402","name":"Chengdu University of Traditional Chinese Medicine","doi-asserted-by":"publisher","award":["QNXZ2018023"],"award-info":[{"award-number":["QNXZ2018023"]}],"id":[{"id":"10.13039\/501100008402","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008402","name":"Chengdu University of Traditional Chinese Medicine","doi-asserted-by":"publisher","award":["XSGG2019008"],"award-info":[{"award-number":["XSGG2019008"]}],"id":[{"id":"10.13039\/501100008402","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2021,1,5]]},"abstract":"<jats:p>Objective. Modern research shows that Haima Duobian pill (HDP) can relieve the kidney yang deficiency syndrome (KYDS), but the mechanism is still unclear. The aim of this work was to study the effects of HDP in a rat model of KYDS. Materials and Methods. The network pharmacology methods were used to predict the therapeutic effects of Haima Duobian pill. Adenine was used to establish the rat model of kidney yang deficiency syndrome. The general physical signs of rats were observed after different doses of Haima Duobian pill (HDP) were given. Serum cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone (T), estradiol (E2), and gonadotropin-releasing hormone (GnRH) levels were determined using enzyme-linked immunosorbent assay (ELISA) kits. Then, the histopathologic changes and sperm activity were detected. Results. HDP could improve the general signs of kidney yang deficiency syndrome rats. After the rats were treated with HDP, the expression of cGMP and E2 was significantly inhibited and the expression of cAMP and T was significantly increased. The pathological damage of testis, epididymis, and seminal vesicle was alleviated, and the sperm activity was improved. Conclusion. For adenine-induced kidney yang deficiency syndrome in rats, HDP had a significant therapeutic effect.<\/jats:p>","DOI":"10.1155\/2021\/6696234","type":"journal-article","created":{"date-parts":[[2021,1,6]],"date-time":"2021-01-06T19:36:35Z","timestamp":1609961795000},"page":"1-13","source":"Crossref","is-referenced-by-count":7,"title":["Effects of Haima Duobian Pill in a Rat Model of Kidney Yang Deficiency Syndrome"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4184-9863","authenticated-orcid":true,"given":"Yijia","family":"Zeng","sequence":"first","affiliation":[{"name":"Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingna","family":"Li","sequence":"additional","affiliation":[{"name":"Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaorui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Ren","sequence":"additional","affiliation":[{"name":"Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinwan","family":"Huang","sequence":"additional","affiliation":[{"name":"Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7278-9368","authenticated-orcid":true,"given":"Jin","family":"Wang","sequence":"additional","affiliation":[{"name":"Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Yuan","sequence":"additional","affiliation":[{"name":"Sichuan Engineering Research Center for Medicinal Animals, Chengdu, Sichuan 610031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3748-4187","authenticated-orcid":true,"given":"Funeng","family":"Geng","sequence":"additional","affiliation":[{"name":"Sichuan Engineering Research Center for Medicinal Animals, Chengdu, Sichuan 610031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2015.12.004"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20153655"},{"issue":"7","key":"3","first-page":"429","article-title":"Basic research and clinical application of infertility caused by spermatogenesis dysfunction","volume":"42","author":"Z. 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