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E. histolytica has the capability to invade host tissue by union of virulence factor Gal\/GalNAc lectin; this molecule induces an adherence-inhibitory antibody response as well as to protect against amebic liver abscess (ALA). The present work showed the effect of the immunization with PE\u0394III-LC3-KDEL3 recombinant protein. In vitro, this candidate vaccine inhibited adherence of E. histolytica trophozoites to HepG2 cell monolayer, avoiding the cytolysis, and in a hamster model, we observed a vaccine-induced protection against the damage to tissue liver and the inhibition of uncontrolled inflammation. PE\u0394III-LC3-KDEL3 reduced the expression of TNF-\u03b1, IL-1\u03b2, and NF-\u03baB in all immunized groups at 4- and 7-day postinfection. The levels of IL-10, FOXP3, and IFN-\u03b3 were elevated at 7 days. The immunohistochemistry assay confirmed this result, revealing an elevated quantity of +IFN-\u03b3 cells in the liver tissue. ALA formation in hamsters immunized was minimal, and few trophozoites were identified. Hence, immunization with PE\u0394III-LC3-KDEL3 herein prevented invasive amebiasis, avoided an acute proinflammatory response, and activated a protective response within a short time. Finally, this recombinant protein induced an increase of serum IgG.<\/jats:p>","DOI":"10.1155\/2021\/6697900","type":"journal-article","created":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T19:35:09Z","timestamp":1616441709000},"page":"1-12","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of the PE\u0394III-LC3-KDEL3 Chimeric Protein of Entamoeba histolytica-Lectin as a Vaccine Candidate against Amebic Liver Abscess"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9714-1974","authenticated-orcid":true,"given":"Sandra L.","family":"Mart\u00ednez-Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"Departamento de Morfolog\u00eda, Centro de Ciencias B\u00e1sicas, Universidad Aut\u00f3noma de Aguascalientes, C. 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