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Periodontitis induced by bacteria especially Gram-negative bacteria is the most prevalent chronic inflammatory disease worldwide. Emerging evidence supported that EZH2 plays a significant role in the inflammatory response of periodontal tissues. However, little information is available regarding the underlying mechanism of EZH2 in periodontitis. This study is aimed at determining the potential role and underlying mechanism of EZH2 in periodontitis. Methods. The protein levels of EZH2, H3K27ME, p-p65, p-IKB, TLR4, MyD88, Runx2, and OCN were examined by western blot assay. Proliferation was evaluated by CCK8 assay. The levels of TNF\u03b1, IL1\u03b2, and IL6 were detected by ELISA assay. Migration was detected by wound healing assay. The distribution of p65 was detected by immunofluorescence. The formation of mineralized nodules was analyzed using alizarin red staining. Results. LPS stimulation significantly promoted EZH2 and H3K27me3 expression in primary human periodontal ligament stem cells (PDLSCs). Targeting EZH2 prevented LPS-induced upregulation of the inflammatory cytokines and inhibition of cell proliferation and migration. Furthermore, EZH2 knockdown attenuated the TLR4\/MyD88\/NF-\u03baB signaling to facilitate PDLSC osteogenesis. Conclusions. Modulation of the NF-\u03baB pathway through the inhibition of EZH2 may offer a new perspective on the treatment of chronic apical periodontitis.<\/jats:p>","DOI":"10.1155\/2021\/7625134","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T19:20:17Z","timestamp":1638386417000},"page":"1-12","source":"Crossref","is-referenced-by-count":35,"title":["EZH2 Regulates Lipopolysaccharide-Induced Periodontal Ligament Stem Cell Proliferation and Osteogenesis through TLR4\/MyD88\/NF-\u03baB Pathway"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6432-669X","authenticated-orcid":true,"given":"Pengcheng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Stomatology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4581-4820","authenticated-orcid":true,"given":"Huan","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Changsha Stomatological Hospital, Hunan University of Traditional Chinese Medicine, Changsha 410004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6954-8790","authenticated-orcid":true,"given":"Zheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Tianjin Stomatological Hospital and Tianjin Key Laboratory of Oral Function Reconstruction, Hospital of Stomatology, Nankai University, Tianjin 300041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3722-377X","authenticated-orcid":true,"given":"Zuomin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345211007855"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1111\/adj.12845"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1159\/000511702"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.05.037"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1007\/s11684-018-0628-x"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1483-7"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2019.0031"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1002\/stem.512"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-016-0288-1"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.440"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00053"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-020-02105-8"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13875"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1186\/s12865-021-00409-9"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.3MA0121-045R"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/1327405"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.930610"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-11093-2_10"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12533"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0219181"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-021113-170408"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1126\/science.1076997"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.2174\/1389203716666150409100233"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1111\/cas.14840"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-03219-w"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1186\/s13041-020-00656-8"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2015.152"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22115920"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.3390\/ani11061528"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2016.160279"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.3390\/cells8121490"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2018.00471"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2020.06.005"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1126\/science.1183021"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2015.11.007"},{"issue":"4","key":"36","first-page":"249","article-title":"Nf-kB transcription factor: role in the pathogenesis of inflammatory, autoimmune, and neoplastic diseases and therapy implications","volume":"152","author":"C. 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