{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T07:33:33Z","timestamp":1790408013826,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T00:00:00Z","timestamp":1630886400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013129","name":"Ministry of SMEs and Startups","doi-asserted-by":"publisher","award":["P0010099"],"award-info":[{"award-number":["P0010099"]}],"id":[{"id":"10.13039\/501100013129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2021,9,6]]},"abstract":"<jats:p>Pueraria lobata (Willd.) Ohwi, known as kudzu, is one of the most popular traditional medicines in Asian countries. It has been widely used as a natural alternative to hormone replacement therapy for treating postmenopausal symptoms. This study aimed to investigate the estrogenic effect of P. lobata extract (PE) against postmenopausal osteoporosis in ovariectomized (OVX) rats. OVX rats were treated with PE (25\u20131600\u2009mg\/kg) for 8 weeks. Biochemical parameters, estradiol, and bone turnover markers (e.g., osteocalcin, C-terminal telopeptide fragment of type I collagen, deoxypyridinoline, and pyridinoline) were measured in plasma samples. In addition, estrogen receptor-alpha (ER-\u03b1) protein expression and morphology of uterine were evaluated. Long-term treatment with PE did not cause liver damage in OVX rats. PE supplementation reduced body weight gain in obese rats with high lipid accumulation induced by ovariectomy. Furthermore, PE exhibited a protective effect against insulin resistance, hyperlipidemia, and hepatic lipid peroxidation. PE treatment increased uterine weight and thickness of the uterine layers in cases of uterus atrophy due to removal of ovaries. The levels of bone turnover markers, which were significantly increased in OVX rats, were decreased by PE treatment. Western blotting analysis showed that ER-\u03b1 protein expression was upregulated in PE-treated rats compared with OVX rats. These results suggest that PE could be a promising alternative functional food for improving menopausal symptoms.<\/jats:p>","DOI":"10.1155\/2021\/7924400","type":"journal-article","created":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T23:50:10Z","timestamp":1630972210000},"page":"1-8","source":"Crossref","is-referenced-by-count":13,"title":["Ameliorative Effects of Pueraria lobata Extract on Postmenopausal Symptoms through Promoting Estrogenic Activity and Bone Markers in Ovariectomized Rats"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2376-1884","authenticated-orcid":true,"given":"Mi-Ra","family":"Lee","sequence":"first","affiliation":[{"name":"Hongcheon Institute of Medicinal Herb, Hongcheon-gun 25142, Gangwon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0167-9794","authenticated-orcid":true,"given":"Bohye","family":"Kim","sequence":"additional","affiliation":[{"name":"Hongcheon Institute of Medicinal Herb, Hongcheon-gun 25142, Gangwon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1450-5515","authenticated-orcid":true,"given":"Yongjun","family":"Lee","sequence":"additional","affiliation":[{"name":"Hongcheon Institute of Medicinal Herb, Hongcheon-gun 25142, Gangwon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0090-3106","authenticated-orcid":true,"given":"Soung-Yong","family":"Park","sequence":"additional","affiliation":[{"name":"Sejun F & B Co. Ltd., Hongcheon-gun 25107, Gangwon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0230-1769","authenticated-orcid":true,"given":"Jae-Hoon","family":"Shim","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Hallym University, Chuncheon 24252, Gangwon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4021-1390","authenticated-orcid":true,"given":"Bong-Hwan","family":"Chung","sequence":"additional","affiliation":[{"name":"Hongcheon Institute of Medicinal Herb, Hongcheon-gun 25142, Gangwon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5239-2638","authenticated-orcid":true,"given":"Seong-Il","family":"Heo","sequence":"additional","affiliation":[{"name":"Hongcheon Institute of Medicinal Herb, Hongcheon-gun 25142, Gangwon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2009.07.061"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.4669"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/689138"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2009.07.009"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2009.05.033"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13622"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1055\/s-0043-108250"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.3109\/13697137.2014.966241"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/5713606"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2012.08.011"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1021\/jf030174a"},{"key":"12","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2020.110734","article-title":"A comprehensive review on Pueraria: insights on its chemistry and medicinal value","volume":"131","author":"S. Wang","year":"2020","journal-title":"Biomedicine & Pharmacotherapy"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2018.05.003"},{"key":"14","article-title":"Puerarin improves the bone micro-environment to inhibit OVX-induced osteoporosis via modulating SCFAs released by the gut microbiota and repairing intestinal mucosal integrity","volume":"132","author":"B. Li","year":"2020","journal-title":"Biomedicine & Pharmacotherapy"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2016.09.024"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/8756-3282(95)98389-5"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.3109\/13697137.2014.933411"},{"issue":"5","key":"18","first-page":"424","article-title":"The laboratory rat as an animal model for osteoporosis research","volume":"58","author":"P. P. Lelovas","year":"2008","journal-title":"Comparative Medicine"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.3177\/jnsv.62.341"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2008.09.006"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1016\/j.chmed.2019.04.004"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2016.08.007"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00504.2009"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1210\/en.2016-1535"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2009.02.067"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.3581"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.3949\/ccjm.75.10.739"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0181175"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejcn.1602748"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2017.06.010"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.090721"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2021\/7924400.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2021\/7924400.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2021\/7924400.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T23:50:15Z","timestamp":1630972215000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ecam\/2021\/7924400\/"}},"subtitle":[],"editor":[{"given":"Sandrina A.","family":"Heleno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,9,6]]},"references-count":31,"alternative-id":["7924400","7924400"],"URL":"https:\/\/doi.org\/10.1155\/2021\/7924400","relation":{},"ISSN":["1741-4288","1741-427X"],"issn-type":[{"value":"1741-4288","type":"electronic"},{"value":"1741-427X","type":"print"}],"subject":[],"published":{"date-parts":[[2021,9,6]]}}}