{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T02:12:52Z","timestamp":1793239972520,"version":"4.5.0"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"vor","delay-in-days":151,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2020M671741"],"award-info":[{"award-number":["2020M671741"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LQ21C170002"],"award-info":[{"award-number":["LQ21C170002"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31630075"],"award-info":[{"award-number":["31630075"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32002185"],"award-info":[{"award-number":["32002185"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>A novel bacteriocin secreted by <jats:italic>Clostridium butyricum<\/jats:italic> ZJU\u2010F1 was isolated using ammonium sulfate fractionation, cation exchange chromatography, affinity chromatography, and reverse\u2010phase high\u2010performance liquid chromatography (RP\u2010HPLC). The bacteriocin, named CBP22, contained 22 amino acids with the sequence PSAWQITKCAGSIAWALGSGIF. Analysis of its structure and physicochemical properties indicated that CBP22 had a molecular weight of 2264.63\u2009Da and a +1 net charge. CBP22 showed activity against <jats:italic>E. col K88<\/jats:italic>, <jats:italic>E. coli ATCC25922<\/jats:italic>, and <jats:italic>S. aureus ATCC26923<\/jats:italic>. The effects and potential mechanisms of bacteriocin CBP22 on the innate immune response were investigated with a lipopolysaccharide\u2010 (LPS\u2010) induced mouse model. The results showed that pretreatment with CBP22 prevented LPS\u2010induced impairment in epithelial tissues and significantly reduced serum levels of IgG, IgA, IgM, TNF\u2010<jats:italic>\u03b1<\/jats:italic>, and sIgA. Moreover, CBP22 treatment increased the expression of the zonula occludens and reduced permeability as well as apoptosis in the jejunum in LPS\u2010treated mice. In summary, CBP22 inhibits the intestinal injury and prevents the gut barrier dysfunction induced by LPS, suggesting the potential use of CBP22 for treating intestinal damage.<\/jats:p>","DOI":"10.1155\/2021\/8032125","type":"journal-article","created":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T14:27:21Z","timestamp":1622557641000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["CBP22, a Novel Bacteriocin Isolated from <i>Clostridium butyricum<\/i> ZJU\u2010F1, Protects against LPS\u2010Induced Intestinal Injury through Maintaining the Tight Junction Complex"],"prefix":"10.1155","volume":"2021","author":[{"given":"Tenghao","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeqing","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengqin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingliang","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3908-7102","authenticated-orcid":false,"given":"Yizhen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5980-1473","authenticated-orcid":false,"given":"Xin","family":"Zong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gtc.2017.08.003"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2018.12.024"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12618"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1523899113"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1155\/2008\/540758"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.08.037"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2018.1534513"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2019.10.016"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.7.3.256"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2009.10.002"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1982.tb06843.x"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/jam.13936"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells10030527"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-016-0061-4"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-011-1084-y"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_201807_15533"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3738"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2020.09.002"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI130976"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.14"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2012-302167"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2014.427"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12876-014-0189-7"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.043257-0"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2011-301501"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-230X-9-95"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2016.01.009"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200115010-00001"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.107.12.3569"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.06.015"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.2525"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2008.08.003"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-013-5246-6"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules17032980"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep17260"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btn392"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3213"},{"key":"e_1_2_10_38_2","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1970.01340280030009"},{"key":"e_1_2_10_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0165-2478(97)02715-6"},{"key":"e_1_2_10_40_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1801151"},{"key":"e_1_2_10_41_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-018-0972-1"},{"key":"e_1_2_10_42_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00564"},{"key":"e_1_2_10_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2018.10.007"},{"key":"e_1_2_10_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-9420-8"},{"key":"e_1_2_10_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1273"},{"key":"e_1_2_10_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2019.12.023"},{"key":"e_1_2_10_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2018.09.001"},{"key":"e_1_2_10_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm009"},{"key":"e_1_2_10_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/415389a"},{"key":"e_1_2_10_50_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir147"},{"key":"e_1_2_10_51_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-13-3588-4_10"},{"key":"e_1_2_10_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.11.006"},{"key":"e_1_2_10_53_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-042617-053238"},{"key":"e_1_2_10_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.09.046"},{"key":"e_1_2_10_55_2","article-title":"miR-150 attenuates LPS-induced acute lung injury via targeting AKT3","volume":"75","author":"Li P.","year":"2019","journal-title":"International Immunopharmacology"},{"key":"e_1_2_10_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.12.1.60-67.2005"},{"key":"e_1_2_10_57_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci9826"},{"key":"e_1_2_10_58_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-020-2754-5"},{"key":"e_1_2_10_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2653"},{"key":"e_1_2_10_60_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02297"},{"key":"e_1_2_10_61_2","article-title":"Serum diamine oxidase as a hemorrhagic shock biomarker in a rabbit model","volume":"9","author":"Zhao L.","year":"2014","journal-title":"PLoS One"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2021\/8032125.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2021\/8032125.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2021\/8032125","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T09:32:09Z","timestamp":1756373529000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2021\/8032125"}},"subtitle":[],"editor":[{"given":"Tomasz","family":"Brzozowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":61,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1155\/2021\/8032125"],"URL":"https:\/\/doi.org\/10.1155\/2021\/8032125","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"2020-07-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"8032125"}}