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To assess the preventive effects of acupuncture at back-shu and front-mu acupoints on rats with restraint water-immersion stress (RWIS)-induced gastric ulcer. Methods. Thirty-six rats were randomly divided into four groups for 10 days of treatment as follows: the normal group received no treatment; the model group received RWIS-induced gastric ulcer; the omeprazole group was administered omeprazole orally every 2 days; and the electroacupuncture group received electroacupuncture at the RN12 and BL21 acupoints every 2 days. After 10 days of treatment, except for the normal group, all rats were induced with gastric ulcer by RWIS for 3\u2009h. The ulcer index (UI), ulcer inhibition rate, and histopathological score were calculated. We determined the levels of tumor necrosis factor (TNF)-\u03b1 and interleukin (IL)-6 in serum, and the activities of myeloperoxidase (MPO), malondialdehyde (MDA), superoxide dismutase (SOD), nitric oxide (NO), and glutathione peroxidase (GSH-Px) in serum and gastric tissues. Protein expression of MyD88, nuclear factor (NF)-\u03baB (p65), and toll-like receptor (TLR) 4 was quantified in gastric tissues. Results. The electroacupuncture and omeprazole groups were equivalent in terms of UI, ulcer inhibition rate, and histopathological score. The serum levels of TNF-\u03b1 and IL-6 were significantly lower in the electroacupuncture group compared with the omeprazole group (<jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>P<\/a:mi>\n                     <\/a:math>\n                  <\/jats:inline-formula>\u2009&lt;\u20090.05). Compared with the model group, there were significant changes in the levels of NO, MPO, GSH-Px, and MDA in all other groups, while the expression of TLR4, MyD88, and NF-\u03baB p65 in gastric tissue decreased significantly in the electroacupuncture group. The expression of TLR4 was substantially lower in the electroacupuncture group compared with the omeprazole group. Conclusion. Acupuncture at back-shu and front-mu acupoints played a role in preventing gastric ulcer by inhibiting extracellular signals, stimulating kinases in serum and gastric tissues, and activating the inhibition of the TLR4 signaling pathway.<\/jats:p>","DOI":"10.1155\/2021\/8214052","type":"journal-article","created":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T04:37:00Z","timestamp":1612931820000},"page":"1-12","source":"Crossref","is-referenced-by-count":7,"title":["Acupuncture at Back-Shu and Front-Mu Acupoints Prevents Gastric Ulcer by Regulating the TLR4\/MyD88\/NF-\u03baB Signaling Pathway"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3161-4913","authenticated-orcid":true,"given":"Li","family":"Li","sequence":"first","affiliation":[{"name":"School of Acupuncture-Moxibustion and Tuina, Changchun University of Chinese Medicine, Changchun 130117, Jilin, China"},{"name":"School of Pharmacy and Medicine, Tonghua Normal University, Tonghua 134002, Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8357-2888","authenticated-orcid":true,"given":"Hao","family":"Zang","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Medicine, Tonghua Normal University, Tonghua 134002, Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Acupuncture-Moxibustion and Tuina, Changchun University of Chinese Medicine, Changchun 130117, Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangshuang","family":"Mu","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiazhen","family":"Cao","sequence":"additional","affiliation":[{"name":"School of Acupuncture-Moxibustion and Tuina, Changchun University of Chinese Medicine, Changchun 130117, Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Qu","sequence":"additional","affiliation":[{"name":"Wuhan Servicebio Technology Co., Ltd., Wuhan 430200, Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4610-4687","authenticated-orcid":true,"given":"Zhaohui","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Acupuncture-Moxibustion and Tuina, Changchun University of Chinese Medicine, Changchun 130117, Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6281-127X","authenticated-orcid":true,"given":"Wei","family":"Qi","sequence":"additional","affiliation":[{"name":"Bao\u2019an Authentic TCM Therapy Hospital, Shenzhen 518101, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2012.03.013"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.54.287"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2014.235"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-4431.2011.00680.x"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/s0149-2918(04)90019-7"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1097\/ccm.0b013e3182758734"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinthera.2020.01.008"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2012.11.020"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.4103\/2141-9248.126604"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/s0891-5849(96)00547-3"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i48.13480"},{"issue":"12","key":"12","first-page":"8","article-title":"Clinical observations on treatment of chronic atrophic gastritis by catgut embedding at combined Shu and Mu Points","volume":"27","author":"J. 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