{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T17:35:07Z","timestamp":1740159307399,"version":"3.37.3"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,11,9]],"date-time":"2021-11-09T00:00:00Z","timestamp":1636416000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005992","name":"Ministry of Education and Science","doi-asserted-by":"publisher","award":["DN 13\/6\/15.12.2017"],"award-info":[{"award-number":["DN 13\/6\/15.12.2017"]}],"id":[{"id":"10.13039\/501100005992","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Inflammation"],"published-print":{"date-parts":[[2021,11,9]]},"abstract":"<jats:p>Known as a degenerative joint disorder of advanced age affecting predominantly females, osteoarthritis can develop in younger and actively working people because of activities involving loading and injuries of joints. Collagenase-induced osteoarthritis (CIOA) in a mouse model allowed us to investigate for the first time its effects on key cytoskeletal structures (meiotic spindles and actin distribution) of ovulated mouse oocytes. Their meiotic spindles, actin caps, and chromatin were analyzed by immunofluorescence. A total of 193 oocytes from mice with CIOA and 209 from control animals were obtained, almost all in metaphase I (M I) or metaphase II (MII). The maturation rate was lower in CIOA (26.42% M II) than in controls (55.50% M II). CIOA oocytes had significantly larger spindles (average 37\u2009\u03bcm versus 25\u2009\u03bcm in controls, <jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>p<\/a:mi>\n                        <a:mo>&lt;<\/a:mo>\n                        <a:mn>0.001<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>), with a proportion of large spindles more than 64% in CIOA versus up to 15% in controls (<jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>p<\/c:mi>\n                        <c:mo>&lt;<\/c:mo>\n                        <c:mn>0.001<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula>). Meiotic spindles were wider in 68.35% M I and 54.90% M II of CIOA oocytes (mean 18.04\u2009\u03bcm\u2009M I and 17.34\u2009\u03bcm\u2009M II versus controls: 11.64\u2009\u03bcm\u2009M I and 12.64\u2009\u03bcm\u2009M II), and their poles were approximately two times broader (mean 6.9\u2009\u03bcm) in CIOA than in controls (3.6\u2009\u03bcm). CIOA oocytes often contained disoriented microtubules. Actin cap was visible in over 91% of controls and less than 20% of CIOA oocytes. Many CIOA oocytes without an actin cap had a nonpolarized thick peripheral actin ring (61.87% of M I and 52.94% of M II). Chromosome alignment was normal in more than 82% in both groups. In conclusion, CIOA affects the cytoskeleton of ovulated mouse oocytes\u2014meiotic spindles are longer and wider, their poles are broader and with disorganized fibers, and the actin cap is replaced by a broad nonpolarized ring. Nevertheless, meiotic spindles were successfully formed in CIOA oocytes and, even when abnormal, allowed correct alignment of chromosomes.<\/jats:p>","DOI":"10.1155\/2021\/8428713","type":"journal-article","created":{"date-parts":[[2021,11,9]],"date-time":"2021-11-09T19:20:06Z","timestamp":1636485606000},"page":"1-12","source":"Crossref","is-referenced-by-count":0,"title":["Osteoarthritis Affects Mammalian Oogenesis: Effects of Collagenase-Induced Osteoarthritis on Oocyte Cytoskeleton in a Mouse Model"],"prefix":"10.1155","volume":"2021","author":[{"given":"Anton Ivanov","family":"Kolarov","sequence":"first","affiliation":[{"name":"Department of Biology, Medical Faculty, Medical University\u2014Sofia, 2 Zdrave Str, 1431 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina Valcheva","family":"Chakarova","sequence":"additional","affiliation":[{"name":"Department of Biology, Medical Faculty, Medical University\u2014Sofia, 2 Zdrave Str, 1431 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina Prodanova","family":"Hadzhinesheva","sequence":"additional","affiliation":[{"name":"Department of Biology, Medical Faculty, Medical University\u2014Sofia, 2 Zdrave Str, 1431 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Venera Pantaleeva","family":"Nikolova","sequence":"additional","affiliation":[{"name":"Department of Biology, Medical Faculty, Medical University\u2014Sofia, 2 Zdrave Str, 1431 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefka Metodieva","family":"Delimitreva","sequence":"additional","affiliation":[{"name":"Department of Biology, Medical Faculty, Medical University\u2014Sofia, 2 Zdrave Str, 1431 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maya Dyankova","family":"Markova","sequence":"additional","affiliation":[{"name":"Department of Biology, Medical Faculty, Medical University\u2014Sofia, 2 Zdrave Str, 1431 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7776-4151","authenticated-orcid":true,"given":"Ralitsa Stefanova","family":"Zhivkova","sequence":"additional","affiliation":[{"name":"Department of Biology, Medical Faculty, Medical University\u2014Sofia, 2 Zdrave Str, 1431 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.2217\/whe.10.38"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2013-203355"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1080\/03009740600902460"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1002\/art.40483"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-018-6114-1"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-018-1627-7"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-017-0411-y"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2017.00349"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.136"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.140382"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1177\/1099800419859091"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0b013e32835a9414"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.22088\/cjim.10.4.383"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1186\/ar3283"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-1215-5_23"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-016-0590-z"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-017-3797-0"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.3109\/08916934.2015.1031889"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1055\/s-0035-1554928"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.218099"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1177\/1721727x1401200212"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.06.025"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003338"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201607062"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2017.00109"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/iox145"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2017.04.009"},{"issue":"3","key":"28","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.cell.2004.10.014","article-title":"Kinetochore orientation in mitosis and meiosis","volume":"119","author":"H. 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