{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:10:48Z","timestamp":1777421448154,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T00:00:00Z","timestamp":1636329600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005317","name":"Kermanshah University of Medical Sciences","doi-asserted-by":"publisher","award":["97684"],"award-info":[{"award-number":["97684"]}],"id":[{"id":"10.13039\/501100005317","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Dentistry"],"published-print":{"date-parts":[[2021,11,8]]},"abstract":"<jats:p>Introduction. Knowledge about the effects of medications, vitamins, and various supplements on orthodontic tooth movement (OTM) is imperative for orthodontists. This study aimed to assess the effect of methotrexate (MTX) injection on OTM in rats. Materials and Methods. Twenty-eight male Wistar rats were randomized into four groups (n\u2009=\u20097). The first molar and central incisor were connected using a nickel-titanium (NiTi) coil spring with a 50\u2009g load in each rat. The two experimental groups received 0.75\u2009mg\/kg and 1.5\u2009mg\/kg MTX, respectively, intraperitoneally for 21 days. The negative control group did not receive any injection and did not undergo orthodontic treatment. The positive control group underwent orthodontic treatment and received 0.9% saline (NaCl) injections for 21 days. All rats were sacrificed with chloroform inhalation after 21 days; their maxilla was resected, and the mean number of Howship\u2019s lacunae, blood vessels, osteoclasts, and resorption lacunae was counted. The reduction in bone volume (bone volume to total volume ratio (BV\/TV)) at the site of the maxillary molar was quantified by microcomputed tomography (micro-CT). Results. OTM, the number of osteoclasts, and the number of blood vessels significantly increased in rats treated with MTX (<jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>P<\/a:mi>\n                        <a:mo>&lt;<\/a:mo>\n                        <a:mn>0.05<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>). However, the increase in the number of Howship\u2019s lacunae and resorption lacunae was not significant (<jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>P<\/c:mi>\n                        <c:mo>&gt;<\/c:mo>\n                        <c:mn>0.05<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula>). Lower BV\/TV in the MTX groups was in agreement with the increased number of osteoclasts. Conclusion. Injection of MTX can significantly increase OTM and decrease root resorption in rats.<\/jats:p>","DOI":"10.1155\/2021\/8451522","type":"journal-article","created":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T18:05:09Z","timestamp":1636394709000},"page":"1-8","source":"Crossref","is-referenced-by-count":4,"title":["Effect of Methotrexate Injection on Orthodontic Tooth Movement: An Experimental Study on Rats"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8167-7632","authenticated-orcid":true,"given":"Amin","family":"Golshah","sequence":"first","affiliation":[{"name":"Department of Orthodontic, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8148-8751","authenticated-orcid":true,"given":"Khaled","family":"Omidi","sequence":"additional","affiliation":[{"name":"Student Research Committee Kermanshah University of Medical Sciences, Kermanshah, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2477-1761","authenticated-orcid":true,"given":"Nafiseh","family":"Nikkerdar","sequence":"additional","affiliation":[{"name":"Department of Maxillofacial Radiology, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9161-8038","authenticated-orcid":true,"given":"Hedaiat","family":"Moradpoor","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1908-8666","authenticated-orcid":true,"given":"Fatemeh","family":"Ghorbani","sequence":"additional","affiliation":[{"name":"Department of Orthodontic, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran"},{"name":"Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1002\/brb3.1506"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.4103\/1735-3327.223618"},{"issue":"6","key":"3","first-page":"8608","article-title":"Effects of icariin on orthodontic tooth movement in rats","volume":"8","author":"Y. Chen","year":"2015","journal-title":"International Journal of Clinical and Experimental Medicine"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/cji015"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-017-9752-1"},{"issue":"2","key":"6","doi-asserted-by":"crossref","first-page":"72","DOI":"10.4103\/2155-8213.100391","article-title":"Drinking coffee may help accelerate orthodontic tooth movement","volume":"3","author":"J. Yi","year":"2012","journal-title":"Dental Hypotheses"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1080\/14740338.2017.1310839"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/s0162-3109(00)00189-2"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcms.2014.01.044"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/58.3.735"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-198466040-00016"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/8756-3282(94)00032-u"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20145"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-69030-x"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000411"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2019.104642"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.3109\/00016357.2015.1090011"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1043\/0003-3219(2004)074<0100:CEDOTM>2.0.CO;2"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.3390\/nu9030255"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajodo.2007.11.035"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1080\/00016357.2017.1365375"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajodo.2017.10.024"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajodo.2018.03.015"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1364\/josaa.1.000612"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2015.12.009"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.25709.3"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-71159-8"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.34172\/joddd.2020.0027"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.922372"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1177\/00220345830620080501"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/22.5.475"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/26.5.483"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/903097"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.5387\/fms.57.11"},{"issue":"504","key":"35","first-page":"504","article-title":"In vivo effects of high-dose methotrexate on bone remodeling in rats","volume":"57","author":"U. Cegie\u00b3a","year":"2005","journal-title":"Pharmacological Reports"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2008.09.014"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780370208"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071592"}],"container-title":["International Journal of Dentistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijd\/2021\/8451522.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijd\/2021\/8451522.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijd\/2021\/8451522.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T18:05:15Z","timestamp":1636394715000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ijd\/2021\/8451522\/"}},"subtitle":[],"editor":[{"given":"Alejandro","family":"Pelaez Vargas","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,11,8]]},"references-count":38,"alternative-id":["8451522","8451522"],"URL":"https:\/\/doi.org\/10.1155\/2021\/8451522","relation":{},"ISSN":["1687-8736","1687-8728"],"issn-type":[{"value":"1687-8736","type":"electronic"},{"value":"1687-8728","type":"print"}],"subject":[],"published":{"date-parts":[[2021,11,8]]}}}