{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T04:42:53Z","timestamp":1778733773011,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,12,30]],"date-time":"2021-12-30T00:00:00Z","timestamp":1640822400000},"content-version":"vor","delay-in-days":363,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004739","name":"Youth Innovation Promotion Association of the Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["2020337"],"award-info":[{"award-number":["2020337"]}],"id":[{"id":"10.13039\/501100004739","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Warburgia ugandensis<\/jats:italic> Sprague (<jats:italic>W. ugandensis<\/jats:italic>), widely distributed in Africa, is a traditional medicinal plant used for the treatment of various diseases including cancer. We intended to evaluate the anticolorectal cancer (CRC) activities of the crude extract from <jats:italic>W. ugandensis<\/jats:italic> (WUD) and reveal the underlying molecular mechanisms of its action. We found that WUD inhibited the proliferation of HT\u201029 and HCT116 cells in a time\u2010 and dose\u2010dependent manner and induced intracellular ROS generation. The inhibitory effect of WUD on the proliferation of HT\u201029 and HCT116 cells could be attenuated by NAC (a ROS scavenger) in a dose\u2010dependent manner. WUD induced G<jats:sub>0<\/jats:sub>\/G<jats:sub>1<\/jats:sub> phase arrest, down\u2010regulated the protein expression of Cyclin D1 via ROS accumulation in HT\u201029 cells. In search of the molecular mechanism involved in WUD\u2010induced Cyclin D1 down\u2010regulation, it was found that WUD can suppress PI3K\/Akt\/GSK3<jats:italic>\u03b2<\/jats:italic> signaling pathway in HT\u201029 cells. Next, it was found that WUD also activated apoptosis, poly\u2010ADP ribose polymerase 1 (PARP1) cleavage and down\u2010regulated pro\u2010caspase 3 in HT\u201029 and HCT116 cells. Besides, WUD decreased the growth of colon tumors <jats:italic>in vivo<\/jats:italic> in the xenograft mouse model. We demonstrated for the first time that ROS and their modulation in the corresponding intracellular signaling could play a significant role in the potential activity of WUD against CRC cells.<\/jats:p>","DOI":"10.1155\/2021\/8807676","type":"journal-article","created":{"date-parts":[[2021,12,30]],"date-time":"2021-12-30T19:05:20Z","timestamp":1640891120000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Inhibition of Growth of Colon Tumors and Proliferation of HT\u201029 Cells by <i>Warburgia ugandensis<\/i> Extract through Mediating G<sub>0<\/sub>\/G<sub>1<\/sub> Cell Cycle Arrest, Cell Apoptosis, and Intracellular ROS Generation"],"prefix":"10.1155","volume":"2021","author":[{"given":"Yongli","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guilin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaocui","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6627-5627","authenticated-orcid":false,"given":"Mingquan","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,30]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clcc.2018.10.002"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21601"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.02.021"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.3126"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.04.034"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytol.2011.02.007"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.4314\/ajtcam.v6i2.57093"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.4314\/thrb.v11i2.45205"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.112742"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/np50009a008"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules14103844"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13659-015-0083-1"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.201500360"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2017.02.067"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules191118993"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2019.03.029"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3735"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-6-24"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602709"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0094508"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.854900"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2013.03.025"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-018-0909-x"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2018.101084"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/5381692"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf500903g"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-17-0407"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929867321666140915142219"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M610185200"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10101559"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109089"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-017-1424-9"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2018.05.010"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2018.03.022"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1902"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-2035-x"},{"key":"e_1_2_10_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5392.1318"},{"key":"e_1_2_10_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.04.001"},{"key":"e_1_2_10_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/35060032"},{"key":"e_1_2_10_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01602-13"},{"key":"e_1_2_10_42_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.746339"},{"key":"e_1_2_10_43_2","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-16-0451"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2021\/8807676.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2021\/8807676.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2021\/8807676","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T18:40:05Z","timestamp":1723056005000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2021\/8807676"}},"subtitle":[],"editor":[{"given":"Fabio","family":"Altieri","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1155\/2021\/8807676"],"URL":"https:\/\/doi.org\/10.1155\/2021\/8807676","archive":["Portico"],"relation":{},"ISSN":["1942-0900","1942-0994"],"issn-type":[{"value":"1942-0900","type":"print"},{"value":"1942-0994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"2021-05-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"8807676"}}