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To investigate the protective function of Bag\u20101, we examined the effects of Bag\u20101L, one isoform of Bag\u20101, in an in vitro cell culture model of lung ischemia\u2010reperfusion injury (LIRI) generated by treatment of A549 cells with hypoxia\/reoxygenation. Overexpression of full\u2010length Bag\u20101L increased the viability of A549 cells and reduced cell apoptosis in response to 6\u2009h of hypoxia\/reoxygenation treatment. Furthermore, Bag\u20101L overexpression enhanced the heat shock protein 70 (HSP70) and Bcl\u20102 protein levels, increased the phosphorylation of AKT, decreased Bax and cleaved caspase\u20103 levels, and was able to overcome cell cycle arrest. These effects were not observed in A549 cells overexpressing a truncated form of Bag\u20101L lacking the \u201cBag domain,\u201d denoted Bag\u20101L\u25b3C. The \u201cBag domain\u201d is the C\u2010terminal 47 amino acids. Taken together, the results of this study suggest that Bag\u20101L overexpression can protect against oxidative stress and apoptosis in an in vitro LIRI model, with a dependence on the Bag domain.<\/jats:p>","DOI":"10.1155\/2021\/8822807","type":"journal-article","created":{"date-parts":[[2021,3,17]],"date-time":"2021-03-17T18:50:07Z","timestamp":1616007007000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Bag\u20101L Protects against Cell Apoptosis in an In Vitro Model of Lung Ischemia\u2010Reperfusion Injury through the C\u2010Terminal \u201cBag\u201d 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