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As the majority of endemic inhabitants exhibit either subclinical or asymptomatic infection which often develops into the active disease state, therapeutic intervention seems to be an important avenue for combating infections by stimulating the natural defense system of infected individuals. With this perspective, the present study focuses on two immunodominant <jats:italic>Leishmania<\/jats:italic> (<jats:italic>L.<\/jats:italic>) <jats:italic>donovani<\/jats:italic> antigens (triosephosphate isomerase and enolase) previously proved to be potent prophylactic VL vaccine candidates, for generating a recombinant chimeric antigen. This is based on the premise that in a heterogeneous population, a multivalent antigen vaccine would be required for an effective response against leishmaniasis (a complex parasitic disease). The resulting molecule rLdT\u2010E chimeric protein was evaluated for its immunogenicity and immunotherapeutic efficacy. A Th1 stimulating adjuvant BCG was employed with the protein which showed a remarkable 70% inhibition of splenic parasitic multiplication positively correlated with boosted Th1 dominant immune response against lethal <jats:italic>L. donovani<\/jats:italic> challenge in hamsters as evidenced by high IFN\u2010<jats:italic>\u03b3<\/jats:italic> and TNF\u2010<jats:italic>\u03b1<\/jats:italic> and low IL\u201010. In addition, immunological analysis of antibody subclass presented IgG2\u2010based humoral response besides considerable delayed\u2010type hypersensitivity and lymphocyte proliferative responses in rLdT\u2010E\/BCG\u2010treated animals. Our observations indicate the potential of the chimera towards its candidature for an effective vaccine against <jats:italic>Leishmania donovani<\/jats:italic> infection.<\/jats:p>","DOI":"10.1155\/2021\/8845826","type":"journal-article","created":{"date-parts":[[2021,2,28]],"date-time":"2021-02-28T03:09:02Z","timestamp":1614481742000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A Chimera of Th1 Stimulatory Proteins of <i>Leishmania donovani<\/i> Offers Moderate Immunotherapeutic Efficacy with a Th1\u2010Inclined Immune Response against Visceral 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