{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T21:07:14Z","timestamp":1789679234020,"version":"4.0.1"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,3,9]],"date-time":"2021-03-09T00:00:00Z","timestamp":1615248000000},"content-version":"vor","delay-in-days":67,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>\n                    <jats:italic>Objective<\/jats:italic>\n                    . This study is aimed at investigating the therapeutic effects of tetrandrine (Tet) on myocardial ischemia reperfusion (I\/R) injury and probe into underlying molecular mechanism.\n                    <jats:italic>Methods<\/jats:italic>\n                    . H9C2 cells were divided into hypoxia\/oxygenation (H\/R) group, H\/R+Tet group, H\/R+Tet+negative control (NC) group, and H\/R+Tet+miR\u2010202\u20105p inhibitor group. RT\u2010qPCR was utilized to monitor miR\u2010202\u20105p and TRPV2 expression, and TRPV2 protein expression was detected via western blot and immunohistochemistry in H9C2 cells. Cardiomyocyte apoptosis was evaluated through detection of apoptosis\u2010related markers and flow cytometry. Furthermore, myocardial enzyme levels were detected by ELISA. Rats were randomly separated into sham operation group, I\/R group, I\/R+Tet group (50\u2009mg\/kg), I\/R+Tet+NC group, and I\/R+Tet+miR\u2010202\u20105p inhibitor group. miR\u2010202\u20105p and TRPV2 mRNA expression was assessed by RT\u2010qPCR. TRPV2 protein expression was detected through western blot and immunohistochemistry in myocardial tissues. Apoptotic levels were assessed via apoptosis\u2010related proteins and TUNEL. Pathological changes were observed by H&amp;E staining. Myocardial infarction size was examined by Evans blue\u2010TCC staining.\n                    <jats:italic>Results<\/jats:italic>\n                    . Abnormally expressed miR\u2010202\u20105p as well as TRPV2 was found in H\/R H9C2 cells and myocardial tissues of I\/R rats, which was ameliorated following Tet treatment. Tet treatment significantly suppressed H\/R\u2010 or I\/R\u2010induced cardiomyocyte apoptosis. ELISA results showed that CK\u2010MB and LDH levels were lowered by Tet treatment in H\/R H9C2 cells and serum of I\/R rats. H&amp;E staining indicated that Tet reduced myocardial injury in I\/R rats. Also, myocardial infarction size was lowered by Tet treatment. The treatment effects of Tet were altered following cotreatment with miR\u2010202\u20105p inhibitor.\n                    <jats:italic>Conclusion<\/jats:italic>\n                    . Our findings revealed that Tet may ameliorate myocardial I\/R damage via targeting the miR\u2010202\u20105p\/TRPV2 axis.\n                  <\/jats:p>","DOI":"10.1155\/2021\/8870674","type":"journal-article","created":{"date-parts":[[2021,3,9]],"date-time":"2021-03-09T18:35:46Z","timestamp":1615314946000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Tetrandrine Ameliorates Myocardial Ischemia Reperfusion Injury through 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