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Pyrethroid insecticides are widely used in many countries for chemical\u2010based control of <jats:italic>Ae. aegypti<\/jats:italic>. Regardless of their efficacy, the constant use of insecticides has induced insecticide resistance mechanisms, such as knockdown resistance (<jats:italic>kdr<\/jats:italic>) in mosquitoes. Sri Lankan Vector Controlling Entities (VCE) have been using a variety of pyrethroid insecticides as the primary approach for dengue control. However, development of any resistance among the <jats:italic>Aedes<\/jats:italic> mosquitoes has been limitedly studied in the country. Therefore, the current study was conducted to evaluate the prevalence of F1534C, V1016G, and S989P mutations among <jats:italic>Ae. aegypti<\/jats:italic> mosquito populations in three dengue endemic high\u2010risk regions of Sri Lanka. <jats:italic>Methodology<\/jats:italic>. Immature (both pupae and larvae) stages of <jats:italic>Ae. aegypti<\/jats:italic> mosquitoes were collected from Colombo, Gampaha, and Kandy districts of Sri Lanka from February 2018 to December 2019. Polymerase Chain Reaction\u2010 (PCR\u2010) based assay for molecular genotyping of mutations was performed to identify the prevalence of <jats:italic>kdr<\/jats:italic> mutations in collected <jats:italic>Ae. aegypti<\/jats:italic> populations, separately. The frequencies of the resistant and susceptible kdr alleles were determined by using the Hardy\u2013Weinberg equilibrium. <jats:italic>Results<\/jats:italic>. The <jats:italic>Ae. aegypti<\/jats:italic> populations from Colombo, Gampaha, and Kandy districts showed 46%, 42%, and 22% of F1534C mutation allele frequencies, along with 15%, 12%, and 6% of V1016G mutation allele frequencies, respectively. The mutation allele frequencies of S989 in Colombo, Gampaha, and Kandy districts were 9.5%, 8.5%, and 4.5%, respectively. The wild\u2010type (PP) genotype remained predominant within all the three districts, whereas the homogenous (QQ) mutation genotype occurred only in minority. The abundance of Q allele frequency in <jats:italic>Ae. aegypti<\/jats:italic> mosquitoes was relatively higher for all the three mutations in Colombo. <jats:italic>Conclusions<\/jats:italic>. The findings clearly indicate that long\u2010term insecticide applications and multiple use of pyrethroids have led to the acquisition of <jats:italic>kdr<\/jats:italic> mutations, leading to the development of insecticide resistance among local <jats:italic>Ae. aegypti<\/jats:italic> populations, especially in the Colombo and Gampaha districts. Therefore, evaluation of the prevalence levels of these <jats:italic>kdr<\/jats:italic> mutations highlights the necessity for shifting towards novel vector control strategies.<\/jats:p>","DOI":"10.1155\/2021\/8874092","type":"journal-article","created":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T20:20:07Z","timestamp":1621887607000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Voltage\u2010Gated Sodium Channel (<i>Vgsc<\/i>) Mutation\u2010Based Pyrethroid Resistance in <i>Aedes aegypti<\/i> Populations of Three Endemic Dengue Risk Areas of Sri Lanka"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9078-1968","authenticated-orcid":false,"given":"Tharaka","family":"Ranathunge","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5687-989X","authenticated-orcid":false,"given":"Lahiru","family":"Udayanga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumudu","family":"Sarasija","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samudra","family":"Karunathilaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shavindhya","family":"Nawarathne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruthra","family":"Rathnarajah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5236-9497","authenticated-orcid":false,"given":"Fathima Fazla","family":"Dulficar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fathima Nafla","family":"Shafi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4107-0024","authenticated-orcid":false,"given":"Ranil S.","family":"Dassanayake","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. 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