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The frequent clinical treatment failure promoted the development of novel antichlamydial agents. Here, we firstly reported a group of pyrroloisoxazolidine\u2010inhibited <jats:italic>C. trachomatis<\/jats:italic> in a dose\u2010dependent manner <jats:italic>in vitro<\/jats:italic>. Among them, compounds 1 and 2 exhibited the strongest inhibitory activity with IC<jats:sub>50<\/jats:sub> values from 7.25 to 9.73\u2009<jats:italic>\u03bc<\/jats:italic>M. The compounds disturbed the whole intracellular life cycle of <jats:italic>C. trachomatis<\/jats:italic>, mainly targeting the middle reticulate body proliferation stages. Besides, the compounds partially inhibited the chlamydial infection by reducing elementary body infectivity at high concentration. Our findings suggest the potential of pyrroloisoxazolidine derivatives as promising lead molecules for the development of antichlamydial agents.<\/jats:p>","DOI":"10.1155\/2021\/8889247","type":"journal-article","created":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T18:35:05Z","timestamp":1615833305000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Inhibitory Activity of Pyrroloisoxazolidine Derivatives against <i>Chlamydia 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