{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T11:19:39Z","timestamp":1762341579931,"version":"3.44.0"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,1,27]],"date-time":"2021-01-27T00:00:00Z","timestamp":1611705600000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005888","name":"Uniwersytet Medyczny w Lodzi","doi-asserted-by":"publisher","award":["503\/1-107-03\/503-11-001-19"],"award-info":[{"award-number":["503\/1-107-03\/503-11-001-19"]}],"id":[{"id":"10.13039\/501100005888","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>The aim of the study was to evaluate the distribution of blood dendritic cells (DCs) in patients with Graves\u2019 orbitopathy (GO) and to assess the influence of methylprednisolone therapy on subsets of peripheral blood mononuclear cells (PBMCs). Peripheral blood DC subsets were analyzed by flow cytometry in patients with active GO (<jats:italic>n<\/jats:italic> = 17), inactive GO (<jats:italic>n<\/jats:italic> = 8), and Graves\u2019 disease (GD) without GO (<jats:italic>n<\/jats:italic> = 8) and controls (<jats:italic>n<\/jats:italic> = 15); additionally, in patients with active GO (<jats:italic>n<\/jats:italic> = 17), analyses were done at three time points, i.e., before methylprednisolone treatment and after 6 weeks and after 12 weeks of the treatment. Percentage of myeloid DCs (mDCs) in PBMC fraction was significantly lower in patients with both active and inactive GO, compared to patients with GD without GO and controls (<jats:italic>p<\/jats:italic> &lt; 0.05). In addition, mDCs were also documented to be an independent factor negatively associated with GO, however without essential differences between active and inactive phases. On the other hand, we did not observe any changes in the percentage of DCs after methylprednisolone therapy (<jats:italic>p<\/jats:italic> &gt; 0.05). In the present study, we have succeeded to firstly demonstrate\u2014according to our knowledge\u2014that blood mDCs are negatively related to GO incidence.<\/jats:p>","DOI":"10.1155\/2021\/8896055","type":"journal-article","created":{"date-parts":[[2021,1,27]],"date-time":"2021-01-27T20:05:16Z","timestamp":1611777916000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Percentage of Myeloid Dendritic Cells in Peripheral Venous Blood Is Negatively Related to Incidence of Graves\u2019 Orbitopathy"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5901-8569","authenticated-orcid":false,"given":"Katarzyna","family":"Wojciechowska-Durczynska","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2999-5964","authenticated-orcid":false,"given":"Katarzyna","family":"Wieczorek-Szukala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8888-7130","authenticated-orcid":false,"given":"Borys","family":"Stefanski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2941-8868","authenticated-orcid":false,"given":"Arkadiusz","family":"Zygmunt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7547-6228","authenticated-orcid":false,"given":"Jan","family":"Stepniak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3045-5669","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Karbownik-Lewinska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8748-3337","authenticated-orcid":false,"given":"Andrzej","family":"Lewinski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,27]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_9_1_2","DOI":"10.1056\/NEJMoa1614949"},{"key":"e_1_2_9_2_2","first-page":"61","article-title":"Dendritic cells in Graves\u2032 disease","volume":"1","author":"Purnamasari D.","year":"2015","journal-title":"Acta Medica Indonesiana"},{"key":"e_1_2_9_3_2","article-title":"Human dendritic cells activated via dectin-1 are efficient at priming Th17, cytotoxic CD8 T and B cell responses","volume":"18","author":"Agrawal S.","year":"2010","journal-title":"PLoS One"},{"doi-asserted-by":"publisher","key":"e_1_2_9_4_2","DOI":"10.1126\/science.283.5405.1183"},{"doi-asserted-by":"publisher","key":"e_1_2_9_5_2","DOI":"10.4049\/jimmunol.173.7.4433"},{"doi-asserted-by":"publisher","key":"e_1_2_9_6_2","DOI":"10.1084\/jem.20061660"},{"doi-asserted-by":"publisher","key":"e_1_2_9_7_2","DOI":"10.1089\/hyb.1988.7.521"},{"doi-asserted-by":"publisher","key":"e_1_2_9_8_2","DOI":"10.1182\/blood.V74.7.2527.2527"},{"doi-asserted-by":"publisher","key":"e_1_2_9_9_2","DOI":"10.1210\/jc.2013-1273"},{"key":"e_1_2_9_10_2","first-page":"46","article-title":"Immune regulatory plasmacytoid dendritic cells selectively accumulate in perithyroidal lymph nodes of patients with Graves disease: implications for the understanding of autoimmunity","volume":"1","author":"Hassan I.","year":"2013","journal-title":"Revista Medico-Chirurgical\u0103\u0306 a Societ\u0103\u0306\u0163\u031cii de Medici \u015f\u0327i Naturali\u015f\u0327ti din Ia\u015f\u0327i"},{"doi-asserted-by":"publisher","key":"e_1_2_9_11_2","DOI":"10.4049\/jimmunol.0904135"},{"doi-asserted-by":"publisher","key":"e_1_2_9_12_2","DOI":"10.1089\/thy.2010.0183"},{"doi-asserted-by":"publisher","key":"e_1_2_9_13_2","DOI":"10.1136\/bjo.2003.035915"},{"doi-asserted-by":"publisher","key":"e_1_2_9_14_2","DOI":"10.1210\/jc.2018-01626"}],"container-title":["Mediators of 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