{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:57:35Z","timestamp":1787169455444,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"vor","delay-in-days":31,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>\n                    <jats:italic>Purpose<\/jats:italic>\n                    . Chronic periodontitis (CP) is a long\u2010lasting inflammatory disease that seriously affects oral health. This study is aimed at investigating the regulatory mechanism of metastasis\u2010associated lung adenocarcinoma transcript 1 (MALAT1) in CP.\n                    <jats:italic>Methods<\/jats:italic>\n                    . Primary human periodontal ligament cells (PDLCs) were treated with\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    lipopolysaccharide (LPS) to establish a CP model. Quantitative real\u2010time PCR (qRT\u2010PCR) was used to measure the expression of MALAT1 and miR\u2010769\u20105p in gingival tissues of patients with CP and LPS\u2010treated PDLCs. Cell viability was detected by 3\u2010(4,5\u2010dimethyl\u20102\u2010thiazolyl)\u20102,5\u2010diphenyl\u20102\u2010H\u2010tetrazolium bromide (MTT) assay. Enzyme\u2010linked immunosorbent assay (ELISA) was used to measure the levels of inflammatory cytokines. The protein levels of caspase\u20103, Bax, Bcl\u20102, and hypoxia\u2010inducible factor (HIF) 3A were determined by western blot assay. Dual\u2010luciferase reporter (DLR) assay was applied to validate the target relationships between miR\u2010769\u20105p and MALAT1\/HIF3A.\n                    <jats:italic>Results<\/jats:italic>\n                    . The expression of MALAT1 and HIF3A was enhanced, and the expression of miR\u2010769\u20105p was reduced in gingival tissues of patients with CP and LPS\u2010treated PDLCs. MALAT1 knockdown promoted cell viability and inhibited inflammation and cell apoptosis in LPS\u2010treated PDLCs. MALAT1 targeted miR\u2010769\u20105p and negatively regulated miR\u2010769\u20105p expression. miR\u2010769\u20105p overexpression promoted cell viability and inhibited inflammation and cell apoptosis in LPS\u2010treated PDLCs. Besides, miR\u2010769\u20105p targeted HIF3A and negatively modulated HIF3A expression. Both miR\u2010769\u20105p inhibition and HIF3A overexpression reversed the inhibitory effects of MALAT1 silencing on LPS\u2010induced PDLC injury in vitro.\n                    <jats:italic>Conclusion<\/jats:italic>\n                    . MALAT1 knockdown attenuated LPS\u2010induced PDLC injury via regulating the miR\u2010769\u20105p\/HIF3A axis, which may supply a new target for CP treatment.\n                  <\/jats:p>","DOI":"10.1155\/2021\/8899863","type":"journal-article","created":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T16:39:23Z","timestamp":1612197563000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Knockdown of MALAT1 Inhibits the Progression of Chronic Periodontitis via Targeting miR\u2010769\u20105p\/HIF3A Axis"],"prefix":"10.1155","volume":"2021","author":[{"given":"Qinchao","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1173-3387","authenticated-orcid":false,"given":"Hanyi","family":"Ge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2013.09.001"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.tcam.2008.02.003"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.12.2187"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/00325481.2018.1396876"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12221"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.02.012"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25015"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/cid.12911"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12693"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-019-0802-9"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12539"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2019.7748"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12700"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.4248\/IJOS11046"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2018.05.008"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2016.125"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28671"},{"key":"e_1_2_8_18_2","first-page":"47","article-title":"Novel findings from determination of common expressed plasma exosomal microRNAs in patients with psoriatic arthritis, psoriasis vulgaris, rheumatoid arthritis, and gouty arthritis","volume":"28","author":"Chen X. M.","year":"2019","journal-title":"Discovery Medicine"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12819"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13590"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29289"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20051173"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.orcp.2020.10.001"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-17-0256"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.4149\/neo_2017_409"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12894"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2019.1699818"},{"key":"e_1_2_8_28_2","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2019.1620256"},{"key":"e_1_2_8_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039878"},{"key":"e_1_2_8_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.139"},{"key":"e_1_2_8_31_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034513487559"},{"key":"e_1_2_8_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2018.07.023"},{"key":"e_1_2_8_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.106600"},{"key":"e_1_2_8_34_2","doi-asserted-by":"publisher","DOI":"10.1080\/03008207.2018.1439480"},{"key":"e_1_2_8_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.27357"},{"key":"e_1_2_8_36_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.23060"},{"key":"e_1_2_8_37_2","doi-asserted-by":"publisher","DOI":"10.18632\/aging.103075"},{"key":"e_1_2_8_38_2","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_202006_21657"},{"key":"e_1_2_8_39_2","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S222836"},{"key":"e_1_2_8_40_2","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2019.1699818"},{"key":"e_1_2_8_41_2","doi-asserted-by":"crossref","DOI":"10.1038\/srep22775","article-title":"miR-429 regulates the transition between hypoxia-inducible factor (HIF)1A and HIF3A expression in human endothelial cells","volume":"6","author":"Janaszak-Jasiecka A.","year":"2016","journal-title":"Scientific Reports"},{"key":"e_1_2_8_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2015.03.046"},{"key":"e_1_2_8_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-24221-5"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2021\/8899863.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2021\/8899863.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2021\/8899863","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T09:20:57Z","timestamp":1735636857000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2021\/8899863"}},"subtitle":[],"editor":[{"given":"Yanming","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1155\/2021\/8899863"],"URL":"https:\/\/doi.org\/10.1155\/2021\/8899863","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"2020-09-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"8899863"}}