{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T18:46:54Z","timestamp":1788029214222,"version":"build-2784847793"},"reference-count":52,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T00:00:00Z","timestamp":1634601600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2021,10,19]]},"abstract":"<jats:p>Background. Diarrhea is one of the tempting symptoms of diseases in the world. In Ethiopian traditional medicine practices, Clerodendrum myricoides is utilized for the treatment of diarrhea without scientific evidence. Objective. This study was aimed to evaluate the antidiarrheal activity of 80% methanol extract and fractions of the leaf of Clerodendrum myricoides in mice. Methods. The crude extract was prepared by maceration in 80% methanol and then fractionated using hexane, chloroform, and distilled water. Antidiarrheal activity was assessed by castor oil-induced diarrhea, enteropooling, and gastrointestinal motility models using onset of diarrhea, number and weight of feces, volume and weight of intestinal contents, and distance travelled by charcoal meal as main parameters. Negative controls received either distilled water or 2% Tween 80 (10\u2009ml\/kg), positive controls received 3\u2009mg\/kg loperamide or 1\u2009mg\/kg atropine, and the test groups received 100, 200, and 400\u2009mg\/kg doses of the extract. Results. The crude extract and chloroform fraction significantly prolonged the onset of diarrhea at 200 and 400\u2009mg\/kg and decreased the number of wet, total, and weight of fresh feces at all tested doses. Hexane fraction has a significant antidiarrheal effect on the onset, number, and weight of feces at 400\u2009mg\/kg. The crude extract and chloroform fraction at all tested doses, as well as aqueous fraction at 200\u2009mg\/kg and 100\u2009mg\/kg, produced significant reduction in volume and weight of intestinal contents. Additionally, hexane fraction showed significant reduction of volume and weight of the intestinal content at 400\u2009mg\/kg. In the gastrointestinal motility test model, both chloroform fraction and crude extract at all tested doses and aqueous fraction at 200\u2009mg\/kg and 400\u2009mg\/kg showed a significant antidiarrheal effect as compared to the negative control. Conclusion. The leaf of Clerodendrum myricoides showed antidiarrheal activity which supports the traditional use.<\/jats:p>","DOI":"10.1155\/2021\/9369173","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T17:50:08Z","timestamp":1634752208000},"page":"1-10","source":"Crossref","is-referenced-by-count":16,"title":["Antidiarrheal Effect of 80% Methanol Extract and Fractions of Clerodendrum myricoides (Hochst.) Vatke (Lamiaceae) Leaf in Swiss Albino Mice"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2315-7495","authenticated-orcid":true,"given":"Getaye Tessema","family":"Desta","sequence":"first","affiliation":[{"name":"Department of Pharmacy, College of Medicine and Health Sciences, Debre Tabor University, Debre Tabor, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1540-3369","authenticated-orcid":true,"given":"Muluken","family":"Adela Alemu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, College of Medicine and Health Sciences, Debre Tabor University, Debre Tabor, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asegedech","family":"Tsegaw","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6634-5971","authenticated-orcid":true,"given":"Tafere Mulaw","family":"Belete","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2284-6704","authenticated-orcid":true,"given":"Baye Yrga","family":"Adugna","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Amhara Regional Health Bureau, Bahir Dar, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.11604\/pamj.2021.38.391.21636"},{"key":"2","volume-title":"Diarrheal Diseases","author":"B. 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