{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:02:26Z","timestamp":1740135746765,"version":"3.37.3"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T00:00:00Z","timestamp":1633305600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81660075","81960087","S2018 078"],"award-info":[{"award-number":["81660075","81960087","S2018 078"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangxi Medical and Health Appropriate Technology Development and Promotion Application Project","award":["81660075","81960087","S2018 078"],"award-info":[{"award-number":["81660075","81960087","S2018 078"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genetics Research"],"published-print":{"date-parts":[[2021,10,4]]},"abstract":"<jats:p>Primary aldosteronism is the most common form of secondary hypertension, and aldosteronoma makes up a significant proportion of primary aldosteronism cases. Aldosteronoma is also called aldosterone-producing adenoma (APA). Although there have been many studies about APA, the pathogenesis of this disease is not yet fully understood. In this study, we aimed to find out the difference of gene expression patterns between APA and nonfunctional adrenocortical adenoma (NFAA) using a weighted gene coexpression network (WGCNA) and differentially expressed gene (DEG) analysis; only the genes that meet the corresponding standards of both methods were defined as real hub genes and then used for further analysis. Twenty-nine real hub genes were found out, most of which were enriched in the phospholipid metabolic process. WISP2, S100A10, SSTR5-AS1, SLC29A1, APOC1, and SLITRK4 are six real hub genes with the same gene expression pattern between the combined and validation datasets, three of which indirectly or directly participate in lipid metabolism including WISP2, S100A10, and APOC1. According to the gene expression pattern of DEGs, we speculated five candidate drugs with potential therapeutic value for APA, one of which is cycloheximide, an inhibitor for phospholipid biosynthesis. All the evidence suggests that phospholipid metabolism may be an important pathophysiological mechanism for APA. Our study provides a new perspective regarding the pathophysiological mechanism of APA and offers some small molecules that may possibly be effective drugs against APA.<\/jats:p>","DOI":"10.1155\/2021\/9553637","type":"journal-article","created":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T23:42:03Z","timestamp":1633390923000},"page":"1-15","source":"Crossref","is-referenced-by-count":0,"title":["In Silico Analysis of the Gene Expression Patterns between Aldosterone-Producing Adenoma and Nonfunctional Adrenocortical Adenoma"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2588-8571","authenticated-orcid":true,"given":"Yongfa","family":"Dai","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Medicine, The First Nanning People\u2019s Hospital, Nanning, Guangxi 530016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2862-0143","authenticated-orcid":true,"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0952-9555","authenticated-orcid":true,"given":"Jie","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, The First Nanning People\u2019s Hospital, Nanning, Guangxi 530016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9721-8086","authenticated-orcid":true,"given":"Jianling","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China"},{"name":"Postdoctoral Mobile Station of Guangxi Medical University, Nanning, Guangxi 530021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.7326\/m20-4873"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/s2213-8587(17)30319-4"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcm.2021.03.005"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2016.01.003"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.ej18-0486"},{"issue":"6018","key":"6","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1126\/science.1198785","article-title":"K+ channel mutations in adrenal aldosterone-producing adenomas and hereditary hypertension","volume":"331","author":"M. Choi","year":"2011","journal-title":"Science (New York, NY)"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2695"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2011.76"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1530\/eje-14-0585"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.4158\/ep-2020-0277"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2010-2143"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.05.026"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.ccr-08-1067"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1093\/biostatistics\/4.2.249"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts034"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-9-559"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506580102"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1126\/science.1132939"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.2147\/ott.s208133"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-2506-0"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.29331"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13544"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.3390\/genes11060601"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.1c00342"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1186\/s13072-019-0283-8"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-019-0245-7"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2021.01.011"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(96)00034-9"},{"issue":"2","key":"31","first-page":"63","article-title":"The effect of adrenocorticotropic hormone (ACTH) and cycloheximide (CHM) on lipid biosynthesis in the rat lung","volume":"29","author":"M. Vasileva-Dimova","year":"1990","journal-title":"Eksperimentalna meditsina i morfologiia"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/s0022-2275(20)34795-7"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(70)90059-7"},{"issue":"5","key":"34","first-page":"551","article-title":"Interaction of the antifolate antibiotic trimethoprim with phosphatidylcholine membranes: a 13C and 31P nuclear magnetic resonance study","volume":"33","author":"G. R. Painter","year":"1988","journal-title":"Molecular Pharmacology"}],"container-title":["Genetics Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/gr\/2021\/9553637.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/gr\/2021\/9553637.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/gr\/2021\/9553637.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T23:42:13Z","timestamp":1633390933000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/gr\/2021\/9553637\/"}},"subtitle":[],"editor":[{"given":"Hafiz Ishfaq","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,10,4]]},"references-count":34,"alternative-id":["9553637","9553637"],"URL":"https:\/\/doi.org\/10.1155\/2021\/9553637","relation":{},"ISSN":["1469-5073","0016-6723"],"issn-type":[{"type":"electronic","value":"1469-5073"},{"type":"print","value":"0016-6723"}],"subject":[],"published":{"date-parts":[[2021,10,4]]}}}