{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T15:11:24Z","timestamp":1774278684153,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,7,9]],"date-time":"2021-07-09T00:00:00Z","timestamp":1625788800000},"content-version":"vor","delay-in-days":189,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>We performed in vitro and in vivo experiments to explore the role of protein kinase C\u2010binding protein 1 (PICK1), an intracellular transporter involved in oxidative stress\u2010related neuronal diseases, in sepsis\u2010related acute kidney injury (AKI). Firstly, PCR, western blotting, and immunohistochemistry were used to observe the expression of PICK1 after lipopolysaccharide\u2010 (LPS\u2010) induced AKI. Secondly, by inhibiting PICK1 in vivo and silencing PICK1 in vitro, we further explored the effect of PICK1 on AKI. Finally, the relationship between PICK1 and oxidative stress and the related mechanisms were explored. We found that the expression of PICK1 was increased in LPS\u2010induced AKI models both in vitro and in vivo. PICK1 silencing significantly aggravated LPS\u2010induced apoptosis, accompanied by ROS production in renal tubular epithelial cells. FSC231, a PICK1\u2010specific inhibitor, aggravated LPS\u2010induced kidney injury. Besides, NAC (N\u2010acetylcysteine), a potent ROS scavenger, significantly inhibited the PICK1\u2010silencing\u2010induced apoptosis. In conclusion, PICK1 might protect renal tubular epithelial cells from LPS\u2010induced apoptosis by reducing excessive ROS, making PICK1 a promising preventive target in LPS\u2010induced AKI.<\/jats:p>","DOI":"10.1155\/2021\/9884297","type":"journal-article","created":{"date-parts":[[2021,7,9]],"date-time":"2021-07-09T21:05:14Z","timestamp":1625864714000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["PICK1 Deficiency Exacerbates Sepsis\u2010Associated Acute Kidney 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