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We previously discovered S100A8\/A9 levels were roughly elevated in the plasma of NPC patients as the promising biomarkers. However, their expressions and underlying functions in NPC tissues are still unknown. In the present study, we analyzed 49 NPC tissues and 20 chronic pharyngitis (CP) tissues. Immunohistochemical staining was performed in different tissues and analyzed by the Mann\u2013Whitney U test statistically. Transwell migration and invasion experiments were further performed to determine S100A8\/A9 effects on NPC. Our results showed that S100A8\/A9 in NPC tissues were significantly higher than those in CP tissues, closely associated with NPC clinical stages. Intriguingly, exogenous S100A8\/A9 protein stimulation could dramatically enhance NPC migration and invasion abilities. In addition, p38 MAPK pathway blockade could diminish the migration and invasion of NPC cells stimulated by S100A8\/A9 proteins. The downstream tumor invasion and migration associated proteins (e.g., MMP7) were also elevated in NPC tissues, consistent with S100A8\/A9 overexpression. Taken together, our present findings suggest that the secreted soluble inflammatory factors S100A8\/A9 might promote cancer migration and invasion via the p38 MAPK signaling pathway along with invasion\/migration associated proteins overexpression in the tumor microenvironment of NPC. This may shed light on the mechanism understanding of NPC prognosis and provide more novel clues for NPC diagnosis and therapy.<\/jats:p>","DOI":"10.1155\/2021\/9913794","type":"journal-article","created":{"date-parts":[[2021,6,23]],"date-time":"2021-06-23T18:50:07Z","timestamp":1624474207000},"page":"1-11","source":"Crossref","is-referenced-by-count":19,"title":["S100A8\/A9 Molecular Complexes Promote Cancer Migration and Invasion via the p38 MAPK Pathway in Nasopharyngeal Carcinoma"],"prefix":"10.1155","volume":"2021","author":[{"given":"Ning","family":"Xu","sequence":"first","affiliation":[{"name":"The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bei-Bei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Research, Yunnan University, Kunming, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia-Ning","family":"Huang","sequence":"additional","affiliation":[{"name":"Wuming Hospital of Guangxi Mediacal University, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue-Min","family":"Yan","sequence":"additional","affiliation":[{"name":"Graduate School of Guangxi Medical University, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Cai","sequence":"additional","affiliation":[{"name":"Graduate School of Guangxi Medical University, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"He","sequence":"additional","affiliation":[{"name":"Graduate School of Guangxi Medical University, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8829-8766","authenticated-orcid":true,"given":"Zi-Jian","family":"Han","sequence":"additional","affiliation":[{"name":"Graduate School of Guangxi Medical University, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1867-3751","authenticated-orcid":true,"given":"Yuan-Jiao","family":"Huang","sequence":"additional","affiliation":[{"name":"Life Science Institute, Guangxi Medical University, Nanning, China"},{"name":"School of Basic Medical Sciences, Guangxi Medical University, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3632-2672","authenticated-orcid":true,"given":"Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Pharmaceutical College, Guangxi Medical University, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0024-5476","authenticated-orcid":true,"given":"Ai-Jun","family":"Jiao","sequence":"additional","affiliation":[{"name":"Pharmaceutical College, Guangxi Medical University, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)90206-k"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2005.06.041"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"issue":"12","key":"4","first-page":"3924","article-title":"Up-regulation of Polo-like Kinase 1 in nasopharyngeal carcinoma tissues: a comprehensive investigation based on RNA-sequencing, gene chips, and in-house tissue arrays","volume":"10","author":"X. 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