{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T02:56:36Z","timestamp":1784170596453,"version":"3.55.0"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,5,31]],"date-time":"2021-05-31T00:00:00Z","timestamp":1622419200000},"content-version":"vor","delay-in-days":150,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Ophthalmology"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>\n                    <jats:italic>Purpose<\/jats:italic>\n                    . To evaluate the presence of ocular surface mucin in patients with atopic and vernal keratoconjunctivitis (AKC\/VKC), we investigated the mRNA expression levels of SAM\u2010pointed domain\u2010containing ETS\u2010like factor (\n                    <jats:italic>SPDEF<\/jats:italic>\n                    ) and mucin\u2010related genes on the ocular surface.\n                    <jats:italic>Methods<\/jats:italic>\n                    . Nineteen patients with AKC or VKC were divided into two groups based on the severity of the disease as determined by their clinical scores for AKC\/VKC: the stable group and the active group. Impression cytology was performed in all patients using filter paper, and the expression levels of\n                    <jats:italic>SPDEF<\/jats:italic>\n                    ,\n                    <jats:italic>MUC1<\/jats:italic>\n                    ,\n                    <jats:italic>MUC4<\/jats:italic>\n                    ,\n                    <jats:italic>MUC5AC<\/jats:italic>\n                    ,\n                    <jats:italic>MUC16<\/jats:italic>\n                    , and eotaxin\u20102 mRNA were determined by real\u2010time reverse\u2010transcription polymerase chain reaction.\n                    <jats:italic>Results<\/jats:italic>\n                    . The results showed that the expression levels of\n                    <jats:italic>SPDEF<\/jats:italic>\n                    and\n                    <jats:italic>MUC5AC<\/jats:italic>\n                    mRNA in the active group were significantly decreased compared with those in the stable group. Furthermore, clinical scores were significantly negatively correlated with the expression levels of\n                    <jats:italic>SPDEF<\/jats:italic>\n                    mRNA and significantly positively correlated with the expression levels of eotaxin\u20102, which is a biomarker for eosinophilic inflammation on the ocular surface. Cluster analysis classified the patients with AKC\/VKC into three clusters, and the stable group was divided into two clusters according to the condition of ocular surface mucin.\n                    <jats:italic>Conclusions<\/jats:italic>\n                    . Ocular surface mucin in patients with AKC\/VKC is altered in accordance with the clinical severity of the disease.\n                  <\/jats:p>","DOI":"10.1155\/2021\/9914786","type":"journal-article","created":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:07:07Z","timestamp":1622506027000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Alterations in Mucin\u2010Associated Gene Expression on the Ocular Surface in Active and Stable Stages of Atopic and Vernal Keratoconjunctivitis"],"prefix":"10.1155","volume":"2021","author":[{"given":"Mariko","family":"Horinaka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8957-3312","authenticated-orcid":false,"given":"Jun","family":"Shoji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Tomioka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukiko","family":"Tonozuka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Inada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1330-5386","authenticated-orcid":false,"given":"Satoru","family":"Yamagami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,31]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2013.07.019"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.11-rai-0335"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2006.02499.x"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/ico.0000000000001904"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/iid3.189"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0021-5155(98)00061-6"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.17-23700"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.3109\/09273949509057807"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1080\/08820538.2016.1238097"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/02713683.2016.1242755"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2013.07.027"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-4835(03)00204-5"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci29176"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI39731"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.03.017"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1080\/02713680500196715"},{"key":"e_1_2_8_17_2","first-page":"2483","article-title":"Alteration in goblet cell numbers and mucin gene expression in a mouse model of allergic conjunctivitis","volume":"42","author":"Kunert K. 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